Hypoxia stress affects the physiological responses, apoptosis and innate immunity of Kuruma shrimp, Marsupenaeus japonicus

Panpan Wang; Hongtao Liu; Sizhe Zhao; Shihao Yu; Shumin Xie; Songsong Hua; Binlun Yan; Chaofan Xing; Huan Gao

doi:10.1016/j.fsi.2022.02.016

Hypoxia stress affects the physiological responses, apoptosis and innate immunity of Kuruma shrimp, Marsupenaeus japonicus

Fish Shellfish Immunol. 2022 Mar:122:206-214. doi: 10.1016/j.fsi.2022.02.016. Epub 2022 Feb 11.

Authors

Panpan Wang¹, Hongtao Liu², Sizhe Zhao³, Shihao Yu³, Shumin Xie³, Songsong Hua³, Binlun Yan¹, Chaofan Xing⁴, Huan Gao⁵

Affiliations

¹ Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, 222005, China; Marine Resource Development Institute of Jiangsu (Lianyungang), Lianyungang, 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, 222005, China; The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm, Nanjing, 210014, China.
² Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, 571126, China.
³ Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, 222005, China.
⁴ Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, 222005, China; Marine Resource Development Institute of Jiangsu (Lianyungang), Lianyungang, 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, 222005, China; The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm, Nanjing, 210014, China. Electronic address: Xingcf@jou.edu.cn.
⁵ Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, 222005, China; Marine Resource Development Institute of Jiangsu (Lianyungang), Lianyungang, 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, 222005, China; The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm, Nanjing, 210014, China. Electronic address: huanmr@163.com.

PMID: 35158069
DOI: 10.1016/j.fsi.2022.02.016

Abstract

For commercial aquatic animals, hypoxia phenomenon often occurs in live transport and aquaculture. In previous studies, much interest has been focused on antioxidant enzyme activities and could not present the complexities. The multifaceted responses, especially considering physiological indexes, histological structure, cell apoptosis, and immune pathways, are still unknown. In this study, we investigated the comprehensive hypoxic responses of Marsupenaeus japonicus. The results showed that the physiological indexes showed time-dependent changes upon hypoxia stress. Hypoxia stress led to significant tissue damage and cell apoptosis in the gill and hepatopancreas. Compared with the control group, the apoptosis index (AI) of the 12 h hypoxic treatment increased significantly (p < 0.05) in the gills and hepatopancreas. Comparative transcriptome analysis identified 900 and 1400 differentially expressed genes (DEGs) in the gill and hepatopancreas, respectively. Several DEGs were related to the lysosome, glycolysis/gluconeogenesis, citrate cycle, and apoptosis, and seven of them were validated using quantitative real-time PCR. This study provided valuable clues to understanding the mechanisms underlying the hypoxic responses of M. japonicus.

Keywords: Apoptosis; Hypoxia stress; Marsupenaeus japonicus; Physiological indexes; Transcriptome.

MeSH terms

Animals
Apoptosis
Hepatopancreas
Hypoxia
Immunity, Innate / genetics
Penaeidae*