Effects of Pore Size and Crosslinking Methods on the Immobilization of Myoglobin in SBA-15

Hengmin Miao; Maosheng Li; Xiaochun Sun; Jikun Xia; Yanqing Li; Jiao Li; Fang Wang; Jiakun Xu

doi:10.3389/fbioe.2021.827552

Effects of Pore Size and Crosslinking Methods on the Immobilization of Myoglobin in SBA-15

Front Bioeng Biotechnol. 2022 Jan 28:9:827552. doi: 10.3389/fbioe.2021.827552. eCollection 2021.

Authors

Hengmin Miao^{1

2}, Maosheng Li^{2

3}, Xiaochun Sun⁴, Jikun Xia^{2

5}, Yanqing Li^{2

6}, Jiao Li¹, Fang Wang², Jiakun Xu²

Affiliations

¹ School of Materials Science and Engineering, Shandong University of Technology, Zibo, China.
² Key Lab of Sustainable Development of Polar Fisheries, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
³ School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
⁴ Marine Science Research Institute of Shandong Province, Qingdao, China.
⁵ College of Marine Science and Biological Engineering, Qingdao University of Science andTechnology, Qingdao, China.
⁶ College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.

Abstract

A series of stable mesoporous silica sieves (SBA-15) with different pore sizes (9.8, 7.2, and 5.5 nm) were synthesized using a hydrothermal method. The resulting mesoporous material was then utilized for protein immobilization using myoglobin (Mb) as the target protein. The effects of pore size and adsorption methods on the immobilization efficiency of Mb in a mesoporous material were studied. The SBA-15 with a pore size of 7.2 nm showed the best loading capacity, reaching 413.8 mg/g. The SBA-15 with a pore size of 9.8 nm showed the highest retained catalytic ability (92.36%). The immobilized enzyme was more stable than the free enzyme. After seven consecutive assay cycles, Mb adsorbed by SBA-15 (Mb/SBA-15) and Mb adsorbed by SBA-15 and crosslinked with glutaraldehyde (Mb/G/SBA-15) retained 36.41% and 62.37% of their initial activity, respectively.

Keywords: SBA-15; catalytic activity; immobilization; myoglobin; reusability.