MicroRNA-506-3p inhibits ovarian cancer metastasis by down-regulating the expression of EZH2

Yue Sun; Chao Meng; Guoyan Liu

doi:10.7150/jca.66959

MicroRNA-506-3p inhibits ovarian cancer metastasis by down-regulating the expression of EZH2

J Cancer. 2022 Jan 4;13(3):943-950. doi: 10.7150/jca.66959. eCollection 2022.

Authors

Yue Sun^{1

2}, Chao Meng^{1

2}, Guoyan Liu^{1

2}

Affiliations

¹ Department of Gynecology and Obstetrics, Tianjin Medical University General Hospital, Tianjin, 300052, China.
² Tianjin Key Laboratory of Female Reproductive Health and Eugenics, Tianjin, 300052, China.

Abstract

Objective: To investigate the role of miR-506-3p in ovarian cancer (OvCa) metastasis. Methods: We overexpressed miR-506-3p in OvCa cells, and cell migration and invasion capacities were assessed in vitro using Transwell assays and wound healing assay. EZH2 is a target of miR-506-3p. We overexpressed and knocked down EZH2 in SKOV3 cells, and assessed its impact on cell migration and invasion. The orthotopic OvCa mouse models were conducted to confirm the role of miR-506-3p in OvCa metastasis. Results: In this research, we found that miR-506-3p reduced EZH2 expression and obviously suppressed the cell migration and invasion in ovarian cancer (OvCa). Moreover, the knockout of EZH2 mimicked the effect of miR-506-3p on invasion and migration, whereas EZH2 overexpression rescued the inhibitory effect of miR-506-3p. The orthotopic OvCa mouse models and clinical cases also confirmed the negative correlation between miR-506-3p and EZH2 in OvCa Conclusions: MiR-506-3p can suppress cell migration and invasion by targeting EZH2 in OvCa. Our study provides evidence supporting miR-506-3p-based therapy in OvCa.

Keywords: EZH2; metastasis; miR-506-3p; ovarian cancer.