Inhibition of Type 2C Protein Phosphatases by ABA Receptors in Abscisic Acid-Mediated Plant Stress Responses

Yeongmok Lee; Suhyeon Heo; Sangho Lee

doi:10.1007/978-1-0716-2156-1_1

Inhibition of Type 2C Protein Phosphatases by ABA Receptors in Abscisic Acid-Mediated Plant Stress Responses

Methods Mol Biol. 2022:2462:1-16. doi: 10.1007/978-1-0716-2156-1_1.

Authors

Yeongmok Lee¹, Suhyeon Heo¹, Sangho Lee²

Affiliations

¹ Department of Biological Sciences, Sungkyunkwan University, Suwon, South Korea.
² Department of Biological Sciences, Sungkyunkwan University, Suwon, South Korea. sangholee@skku.edu.

PMID: 35152376
DOI: 10.1007/978-1-0716-2156-1_1

Abstract

Abscisic acid (ABA) signaling pathway constitutes a key stress response mechanism in plants. Alternate interaction of a type 2C protein phosphatase (PP2C) with either an ABA receptor or a SNF1-related protein kinase 2 (SnRK2) is at the core of regulation of the ABA signaling pathway. Binding of an ABA receptor to a PP2C inhibits the enzymatic activity of the PP2C. Presence of multiple paralogs of ABA receptors and PP2Cs necessitates development of an inhibition assay. Here, we describe how to prepare the recombinant proteins of ABA receptors and PP2Cs for the inhibition assay and how to perform a colorimetry-based inhibition assay.

Keywords: ABA receptor; ABA signaling; ABA-dependent inhibition; Abscisic acid; PP2C; Phosphatase inhibition assay; pNPP.

MeSH terms

Abscisic Acid / metabolism
Arabidopsis Proteins* / genetics
Arabidopsis Proteins* / metabolism
Arabidopsis* / metabolism
Gene Expression Regulation, Plant
Phosphoprotein Phosphatases / genetics
Phosphoprotein Phosphatases / metabolism
Protein Phosphatase 2C / genetics
Protein Phosphatase 2C / metabolism

Substances

Arabidopsis Proteins
Abscisic Acid
Phosphoprotein Phosphatases
Protein Phosphatase 2C