Turn-off fluorescence of nitrogen and sulfur carbon quantum dots as effective fluorescent probes for determination of imatinib. Application to biological fluids

Abstract

Nitrogen and sulfur carbon quantum dots(N,S-CQDs) as effective fluorescent nanoprobes were synthesized through one-step-hydrothermal method using thiosemicarbazide (as nitrogen and sulfur source) and citric acid (as carbon source). The highly fluorescent N,S-CQDs were subjected to various characterization techniques. The fluorescence of the synthesized N,S-CQDs is characterized by maximum fluorescence emission at 415 nm after excitation at 345 nm and a high quantum yield of 0.58. The native N,S-CQDs fluorescence is quantitatively quenched upon addition of imatinib (IMA), so they are used for its spectrofluorimetric determination in its pharmaceutical formulations and biological fluids. Under optimal conditions, N,S-CQDs exhibited a "turn-off" fluorescence response to IMA over the range of 1.0 to 15.0 µg/mL with a limit of quantification of 0.42 µg/mL and a lower detection limit of 0.14 µg/mL. Stern-Volmer equation was used to study the mechanism of quenching and it was found to occur through static quenching mechanism. The method was extended to the in-vitro determination of the drug in spiked human urine and plasma samples and the percent recoveries were satisfactory.

Keywords: Biological fluids; Imatinib; Nitrogen and sulfur-carbon quantum dots; Static quenching.