Microplastics accumulation in mangroves increasing the resistance of its colonization Vibrio and Shewanella

Baoyi Tan; Yibin Li; Huifeng Xie; Zhenqing Dai; Chunxia Zhou; Zhong-Ji Qian; Pengzhi Hong; Yanqiu Liang; Lei Ren; Shengli Sun; Chengyong Li

doi:10.1016/j.chemosphere.2022.133861

Microplastics accumulation in mangroves increasing the resistance of its colonization Vibrio and Shewanella

Chemosphere. 2022 May:295:133861. doi: 10.1016/j.chemosphere.2022.133861. Epub 2022 Feb 8.

Authors

Baoyi Tan¹, Yibin Li², Huifeng Xie², Zhenqing Dai³, Chunxia Zhou⁴, Zhong-Ji Qian², Pengzhi Hong⁴, Yanqiu Liang⁵, Lei Ren⁶, Shengli Sun², Chengyong Li⁷

Affiliations

¹ College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China.
² School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, China.
³ Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518108, China. Electronic address: daizq@gdou.edu.cn.
⁴ College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518108, China.
⁵ School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518108, China.
⁶ Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518108, China; College of Agriculture, Guangdong Ocean University, Zhanjiang, 524088, China.
⁷ School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518108, China. Electronic address: cyli@gdou.edu.cn.

PMID: 35149013
DOI: 10.1016/j.chemosphere.2022.133861

Abstract

The enrichment of various pollutants in mangrove has attracted widespread attention. Especially, microplastics accumulation in mangrove may provide a more challenging ecological colonization site by enriching pollutants, thus affecting the change of microplastics antibiotic resistance and increasing the risk of antibiotic failure. Herein, the antibiotic-resistant of microplastics and sediment from mangrove were investigated. The results show that isolates are mainly colonized by Vibrio parahemolyticus (V. parahemolyticus), Vibrio alginolyticus (V. alginolyticus), and Shewanella. 100% mangrove microplastics isolates are resistant to chloramphenicol, cefazolin, and tetracycline, especially amoxicillin clavulanate and ampicillin. Meanwhile, the multiple antibiotics resistance (MAR) indexes of V. parahaemolyticus, Shewanella, and V. alginolyticus in mangrove microplastics are 0.72, 0.77, and 0.77, respectively, which are far higher than the MAR index standard (0.2) and that of mangrove sediment isolates. Furthermore, compared with V. parahaemolyticus isolated from the same mangrove microplastics, Shewanella and V. alginolyticus show stronger drug resistance. It should be noted that there is a closely related relationship between the type of microplastics and the antibiotics resistance of isolated bacteria. For the antibiotics sensitivity test of norfloxacin, streptomycin, amoxicillin, and chloramphenicol, V. parahaemolyticus have the lower antibiotics resistance than that of V. alginolyticus isolated from the same mangrove microplastics. However, Vibrio isolated from PE has stronger antibiotics resistance. Results reveal that mangrove may be one of the potential risks for emergence and spread of bacterial antibiotics-resistant and multidrug-resistant, and microplastic biofilms may act as promoters of bacterial antibiotic resistance.

Keywords: Antibiotic resistance; Mangrove; Microplastics; Shewanella; Vibrio.

MeSH terms

Anti-Bacterial Agents / pharmacology
Drug Resistance, Bacterial
Microbial Sensitivity Tests
Microplastics
Plastics
Shewanella*
Vibrio parahaemolyticus*

Substances

Anti-Bacterial Agents
Microplastics
Plastics