Insights into the molecular basis of hypergravity-induced root growth phenotype in bread wheat (Triticum aestivum L.)

Malarvizhi Sathasivam; Basavalingayya K Swamy; Kushagra Krishnan; Rita Sharma; Spurthi N Nayak; D S Uppar; Ravikumar Hosamani

doi:10.1016/j.ygeno.2022.110307

Insights into the molecular basis of hypergravity-induced root growth phenotype in bread wheat (Triticum aestivum L.)

Genomics. 2022 Mar;114(2):110307. doi: 10.1016/j.ygeno.2022.110307. Epub 2022 Feb 7.

Authors

Malarvizhi Sathasivam¹, Basavalingayya K Swamy¹, Kushagra Krishnan², Rita Sharma³, Spurthi N Nayak¹, D S Uppar⁴, Ravikumar Hosamani⁵

Affiliations

¹ Institute of Agricultural Biotechnology (IABT), University of Agricultural Sciences, Dharwad, Karnataka 580005, India.
² School of Computational & Integrative Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
³ Department of Biological Sciences, Birla Institute of Technology and Science, Pilani Campus, Rajasthan 333031, India.
⁴ Department of Seed Science and Technology, University of Agricultural Sciences, Dharwad, Karnataka 580005, India.
⁵ Institute of Agricultural Biotechnology (IABT), University of Agricultural Sciences, Dharwad, Karnataka 580005, India. Electronic address: hosamanirr@uasd.in.

PMID: 35143884
DOI: 10.1016/j.ygeno.2022.110307

Abstract

Hypergravity is a condition where the force of gravity exceeds that on the surface of the Earth and can be simulated by centrifugation. Previously, a significant increase in root growth phenotype was observed when wheat seeds were exposed to hypergravity (10 g for 12 h). In the present study, we investigated the molecular basis of this change through root transcriptome. The data revealed a total of 3765 up-regulated and 2102 down-regulated transcripts in response to hypergravity. GO enrichment analysis revealed hormonal responses, cell division, and cell-wall-related terms were significantly enriched in hypergravity. The increased isoform level expression of transcripts involved in auxin biosynthesis, transport, and signaling was observed. Further, enhanced expression of cell division transcripts and down-regulation of cell number regulator genes suggests rapid cell division. Overexpression of cellulose and hemicellulose biosynthesis transcripts suggests demand for cell-wall constituents. Collectively, this study identified candidate genes associated with hypergravity-induced enhanced root growth.

Keywords: Hypergravity; RNA-Seq; Root; Wheat.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bread
Cell Wall / genetics
Cell Wall / metabolism
Gene Expression Regulation, Plant
Hypergravity*
Phenotype
Triticum* / metabolism