Glycans are usually fluorescently tagged by reductive amination for analytic tools. However, free reducing glycan regeneration is sometimes important and necessary for further structural or functional studies. Here, we introduce a new method for efficiently removing fluorescent tags from glycoconjugates by a simple treatment with Oxone. This method is proven to be fast and general after being tested on a series of common saccharides and widely used tags. We successfully achieved N-glycopeptide synthesis by using the regenerated glycans.