Pulsed infrared stimulation evoked electrical potential in mouse vestibular system

Weitao Jiang; Zihan Wang; Shijie Xiao; Dingxuan Zeng; Zhuli Wu; Cheng Peng; Fangyi Chen

doi:10.1016/j.neulet.2022.136510

Pulsed infrared stimulation evoked electrical potential in mouse vestibular system

Neurosci Lett. 2022 Apr 1:775:136510. doi: 10.1016/j.neulet.2022.136510. Epub 2022 Feb 5.

Authors

Weitao Jiang¹, Zihan Wang², Shijie Xiao¹, Dingxuan Zeng¹, Zhuli Wu¹, Cheng Peng³, Fangyi Chen⁴

Affiliations

¹ Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China.
² R＆D Division, Shengqiang Technology Co. Ltd., Longgang District, Shenzhen 518055, China.
³ Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China; Department of Biology, Brain Research Center, Southern University of Science and Technology, Shenzhen 518055, China. Electronic address: pengc@sustech.edu.cn.
⁴ Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China; Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institution, Shenzhen 518055, China; Shenzhen Key laboratory of Smart Healthcare Engineering, China. Electronic address: chenfy@sustech.edu.cn.

PMID: 35134492
DOI: 10.1016/j.neulet.2022.136510

Abstract

To improve accuracy of VsEP and avoid the inherent limitation of mechanical vibration, we designed an infrared optical stimulation approach to stimulate mouse vestibular system and measured the evoked potential. IR pulses (1871 nm, 30 pps and 100 μs pulse width) were delivered to mice with different vestibular dysfunction levels and the evoked potential was recorded. The result suggests that the amplitude and latency of the IR-evoked potential (IR-VsEP) were significantly associated with vestibular function integrity. Immunofluorescence staining confirmed that magnitude of IR-VsEP decreased was consistent with the loss of HCs. Micro-CT imaging revealed that the optical fiber was orientating towards the vestibular system. Taken together, we found that: 1) IR stimulation can generate VsEP evoked potential in vestibular system (IR-VsEP), which can be potentially used for vestibular function evaluation; 2) intact HCs and fully functional synaptic transmission are crucial for efficient IR-induced vestibular system stimulation.

Keywords: Hair cell; Infrared neuron stimulation; Vestibular evoked potential; Vestibular function evaluation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Evoked Potentials
Vestibular System*
Vestibule, Labyrinth* / physiology
Vibration