Sphingobacterium bovistauri sp. nov., Isolated from the Faeces of Bos Taurus

Abstract

A novel bacterium designated WQ 366 ^T was isolated from the faeces of Bos taurus, foraging on the slopes of the Baima Snow Mountain in Yunnan, China. The isolate grew optimally at 30 ℃ and pH 7.0-8.0 without NaCl. The cells were Gram-stain-negative, aerobic, rod-shaped, non-gliding, catalase-positive, and produced yellow color colonies on Columbia Agar. A polyphasic study was applied to clarify its taxonomic position through 16S rRNA gene and genome sequence analysis, and other extensive biological typing. Phylogenetic analysis revealed that the isolate was affiliated to the genus Sphingobacterium and its 16S rRNA gene sequence was closely related to Sphingobacterium bovisgrunnientis YK2 ^T (97.3%), Sphingobacterium composti T5-12 ^T (96.4%), and Sphingobacterium cavernae 5.0403-2 ^T (96.4%). The calculated whole genome average nucleotide identity (ANI) and the digital DNA-DNA hybridization values between strain WQ 366 ^T and the three related strains were 78.3, 78.6, 73.9 and 21.2, 21.2, 21.0%, respectively. The predominant fatty acids (>10%) were iso-C_15:0, iso-C_17:0 3-OH, Summed Feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), and Summed feature 9 (iso-C_17:1 ω9c and 10-methyl C_16:0). The main polar lipids were PE, GPL, GL, and PL. MK-7 was the major menaquinone. The genome size and the G + C content of WQ 366 ^T was 4.1 Mb and 34.6%, respectively. All these results indicated that strain WQ 366 ^T represents a novel species of the Sphingobacterium genus. Therefore, the name Sphingobacterium bovistauri sp. nov. is proposed, and the type strain is WQ 366 ^T (= CCTCC AA 2020029 ^T = KCTC 82395 ^T).