Abstract
An unidentified open reading frame, 248 or 255 amino acids in length, on the maize chloroplast DNA fragment Bam5 was sequenced. It encodes a protein which contains a high proportion of hydrophilic amino acids, of which 22% are hydroxylated, interrupted by hydrophobic domains. A synthetic peptide corresponding to a hydrophilic sequence was used to generate antibodies. Western blots of photosystem I and II complexes prepared from maize and spinach thylakoids indicate that the psbG gene product is a membrane-associated protein of the photosystem II complex that migrates as a 24-kDa species on polyacrylamide gel electrophoresis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Chlorophyll / analysis*
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Chlorophyll / genetics
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Chloroplasts / analysis
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DNA / analysis
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Electrophoresis, Polyacrylamide Gel
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Light-Harvesting Protein Complexes
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Molecular Weight
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Photosynthetic Reaction Center Complex Proteins
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Photosystem I Protein Complex
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Photosystem II Protein Complex
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Plant Proteins / analysis*
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Plant Proteins / genetics
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Zea mays
Substances
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Light-Harvesting Protein Complexes
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Photosynthetic Reaction Center Complex Proteins
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Photosystem I Protein Complex
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Photosystem II Protein Complex
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Plant Proteins
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Chlorophyll
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DNA