Hydrogen polysulfides (H2Sn) as an important member of reactive sulfur species is closely relevant to many physiological functions in redox homeostasis and metabolism. Dual-channel monitor the changes of H2Sn level in vivo is highly desired. Herein we design a simple ratiometric fluorescent probe based on flavone skeleton for highly selective detection of H2Sn. The probe HF-NA-MC bearing 2-fluoro-5-nitrobenzoic acid group inhibited the intramolecular ESIPT process, which show the blue fluorescence of adjacent naphthalene unit. In the presence of H2Sn, the enol form of probe is converted to conjugated keto form, resulted in a 90 nm red-shift of fluorescence emission from 450 nm to 540 nm. The ratiometric intensity (I540/I450) of the probe exhibits a good linear relationship toward H2Sn in the range of 0-120 μM, and the detection limit is estimated to be 0.63 μM. The ratiometric fluorescent probe shows high specificity and anti-interference ability for H2Sn over other related reactive sulfur species. The probe HF-NA-MC shows promising outlook and could be applied to the confocal imaging of H2Sn by dual emission channels in Hela cells.
Keywords: Cell imaging; ESIPT; Flavone skeleton; Hydrogen polysulfides; Ratiometric fluorescent.
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