LpNAC6 reversely regulates the alkali tolerance and drought tolerance of Lilium pumilum

Hao Yan; Bin Liu; Ying Cui; Ying Wang; Shaoying Sun; Jingwen Wang; Mengmeng Tan; Yiping Wang; Yanni Zhang

doi:10.1016/j.jplph.2022.153635

LpNAC6 reversely regulates the alkali tolerance and drought tolerance of Lilium pumilum

J Plant Physiol. 2022 Mar:270:153635. doi: 10.1016/j.jplph.2022.153635. Epub 2022 Feb 2.

Authors

Hao Yan¹, Bin Liu¹, Ying Cui¹, Ying Wang¹, Shaoying Sun¹, Jingwen Wang¹, Mengmeng Tan¹, Yiping Wang¹, Yanni Zhang²

Affiliations

¹ College of Landscape Architecture, Northeast Forestry University, Harbin, 150040, China.
² College of Landscape Architecture, Northeast Forestry University, Harbin, 150040, China. Electronic address: zhangyanni808@126.com.

PMID: 35124291
DOI: 10.1016/j.jplph.2022.153635

Abstract

NAC transcription factors have multiple biological functions in plants. In this study, a new NAC transcription factor, LpNAC6, was cloned from Lilium pumilum, and its salt and drought resistance functions were identified. We treated LpNAC6 transgenic tobacco plants with different intensities of alkali and drought stress. Results showed that LpNAC6 transgenic tobacco had enhanced alkali tolerance, but decreased drought tolerance. Antioxidant enzyme (SOD, POD, CAT) activity, chlorophyll content, proline content, and photosynthetic capacity of transgenic tobacco were significantly higher than those of wild-type tobacco, while the contents of MDA, H₂O₂, and O₂^- were significantly lower than those of wild-type tobacco. The expression level of stress-related genes in transgenic tobacco increased significantly, and the alkali tolerance was enhanced, but the opposite was true under drought stress. Our findings suggest that LpNAC6 has a reverse regulatory effect on alkaline and drought tolerance in plants, which is of great significance for plant screening and stress tolerance regulation of transgenic plants in arid saline-alkali land.

Keywords: Abiotic stress; Lilium pumilum; LpNAC6 NAC transcription factor; Reverse regulation.