Self-enhanced electrochemiluminescence of luminol induced by palladium-graphene oxide for ultrasensitive detection of aflatoxin B1 in food samples

Mengke Xia; Xue Yang; Tianhui Jiao; Munetaka Oyama; Quansheng Chen; Xiaomei Chen

doi:10.1016/j.foodchem.2022.132276

Self-enhanced electrochemiluminescence of luminol induced by palladium-graphene oxide for ultrasensitive detection of aflatoxin B₁ in food samples

Food Chem. 2022 Jul 1:381:132276. doi: 10.1016/j.foodchem.2022.132276. Epub 2022 Jan 30.

Authors

Mengke Xia¹, Xue Yang¹, Tianhui Jiao¹, Munetaka Oyama², Quansheng Chen³, Xiaomei Chen⁴

Affiliations

¹ College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
² Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8520, Japan.
³ College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China. Electronic address: chenqs@jmu.edu.cn.
⁴ College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, China. Electronic address: xmchen@jmu.edu.cn.

PMID: 35121311
DOI: 10.1016/j.foodchem.2022.132276

Abstract

In this work, a novel and credible electrochemiluminescence immunoassay (ECLIA) was constructed for the ultrasensitive and highly selective detection of aflatoxin B₁ (AFB₁). Amino-functionalized 3D graphene hydrogel (NGH) served as the ECL platform with the self-enhanced ECL of luminol-palladium-graphene oxide (lum-Pd-GO) acting as a marker for the antibodies against AFB₁. Pd-GO was synthesized by a self-redox method; it promotes the formation of reactive oxygen species, which are important to the ECL of luminol, from dissolved oxygen. The π-π conjunction between luminol and GO shortens their electron transfer distance, resulting in an amplified ECL signal (∼8.5 times larger than conventional luminol ECL). Moreover, 3D NGH, with its good conductivity, large surface area, and sufficient amino groups, was used to anchor gold nanoparticles (AuNPs), which subsequently immobilized bovine serum albumin (BSA)-AFB₁ through Au-S bonds. The resultant, competitive ECLIA gave a relative low detection limit of 5 × 10^-3 μg kg^-1 and exhibited a broad linear relationship over the range of 0.05-50 μg kg^-1. Finally, the proposed ECLIA was successfully used to analyze AFB₁ contents in food samples. ECLIA: electrochemiluminescence immunoassay; AFs: Aflatoxins; HPLC: high-performance liquid chromatography.

Keywords: Aflatoxin B(1); Amino-functionalized graphene hydrogel; Electrochemiluminescence; Luminol; Palladium–graphene oxide.

MeSH terms

Aflatoxin B1
Biosensing Techniques* / methods
Electrochemical Techniques / methods
Gold / chemistry
Graphite* / chemistry
Limit of Detection
Luminescent Agents* / chemistry
Luminescent Measurements / methods
Luminol / chemistry
Metal Nanoparticles* / chemistry
Palladium / chemistry

Substances

Luminescent Agents
graphene oxide
Luminol
Palladium
Gold
Graphite
Aflatoxin B1