Production of Influenza H5 Vaccine Oligomers in Plants

Hoang Trong Phan; Udo Conrad

doi:10.1007/978-1-0716-2168-4_5

Production of Influenza H5 Vaccine Oligomers in Plants

Methods Mol Biol. 2022:2465:97-107. doi: 10.1007/978-1-0716-2168-4_5.

Authors

Hoang Trong Phan¹, Udo Conrad²

Affiliations

¹ Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
² Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany. conradu@ipk-gatersleben.de.

PMID: 35118617
DOI: 10.1007/978-1-0716-2168-4_5

Abstract

The transient expression of veterinary vaccines in plants is a promising tool because of its low cost connected with a practically unlimited scale-up. To achieve these goals, two major challenges, high immunogenicity of vaccines and minimal of down-stream processing cost, have to be overcome. Here we present and discuss protocols enabling to generate highly immunogenic H5 influenza candidate vaccines as H5 oligomers, by transient expression in Nicotiana benthamiana plants and to perform analytical experiments as Western blot, ELISA, and hemagglutination and hemagglutination inhibition assays.

Keywords: H5 oligomers; Influenza vaccine; Plant-derived hemagglutinin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus
Humans
Influenza A Virus, H5N1 Subtype*
Influenza Vaccines*
Influenza, Human*
Vaccines, Synthetic

Substances

Antibodies, Viral
Hemagglutinin Glycoproteins, Influenza Virus
Influenza Vaccines
Vaccines, Synthetic