Detection of aflatoxin B1 with a new label-free fluorescence aptasensor based on PVP-coated single-walled carbon nanohorns and SYBR Gold

Abstract

This paper describes a novel fluorescence label-free aptasensor to detect aflatoxin B₁ (AFB₁) by utilizing SYBR Gold, aptamer, and single-walled carbon nanohorns (SWCNHs). In the presence of AFB₁, the conformation of AFB₁-specific aptamer went through and the spatial structure of this specific aptamer was transformed accordingly. Due to the resistance of the transformed aptamer when adsorbed on the surface of SWCNHs, the protection of the fluorescence of SYBR Gold was accomplished. Consequently, concentrations of AFB₁ showed a strong association with fluorescence intensity. The detection limit (LOD) of AFB₁ was 1.89 ng/mL, while the linear range was 5-200 ng/mL and fluorescence intensity satisfactorily correlated (R² = 0.9919) with the logarithm of AFB₁ concentration.

Keywords: Aflatoxin B1; Aptamer; SYBR Gold; Single-walled carbon nanohorns.