MicroRNA-106a suppresses prostate cancer proliferation, migration and invasion by targeting tumor-derived IL-8

Pengfei Shen; Guangxi Sun; Peng Zhao; Jindong Dai; Xingming Zhang; Jinge Zhao; Sha Zhu; Junru Chen; Ronggui Tao; Jiyu Yang; Hao Zeng

doi:10.21037/tcr.2020.03.70

MicroRNA-106a suppresses prostate cancer proliferation, migration and invasion by targeting tumor-derived IL-8

Transl Cancer Res. 2020 May;9(5):3507-3517. doi: 10.21037/tcr.2020.03.70.

Authors

Pengfei Shen^{1

2

3}, Guangxi Sun^{1

2}, Peng Zhao^{1

2}, Jindong Dai^{1

2}, Xingming Zhang^{1

2}, Jinge Zhao^{1

2}, Sha Zhu^{1

2}, Junru Chen^{1

2}, Ronggui Tao^{1

2}, Jiyu Yang^{1

2}, Hao Zeng^{1

2}

Affiliations

¹ Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
² Institute of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
³ National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China.

Abstract

Background: Tumor-derived interleukin-8 (IL-8) promotes tumorigenesis and progression of prostate cancer (PCa). MicroRNAs (miRNAs) are noncoding regulatory RNAs and their dysregulation is known to be implicated in carcinogenesis. However, the post-transcriptional mechanism of IL-8 via miRNAs is not fully understood. This study was intended to investigate whether miR-106a could affect the progression of PCa via targeting IL-8 or not.

Methods: Using bioinformatics analysis, we postulated that IL-8 might be post-transcriptionally regulated by miR-106a. This was validated by dual reporter gene assays that miR-106a could bind to the predicted site of IL-8 mRNA. To determine the biological effects of miR-106a on PCa cells (PC-3 and DU145), MTT, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), migration and invasion assays were performed.

Results: We found that miR-106a was barely expressed in PCa cells, whereas IL-8 was aberrantly upregulated. Elevated miR-106a could reduce IL-8 expression by directly binding the 3'-UTR of IL-8. Overexpression of miR-106a in PCa cells triggered cell apoptosis and suppressed cell proliferation, migration, and invasion.

Conclusions: This research showed that miR-106a could function as a tumor-suppressor by decreasing IL-8 levels in PCa.

Keywords: Prostate cancer (PCa); interleukin-8 (IL-8); miR-106a; progression.