Interactomes: Experimental and In Silico Approaches

Luíza Lane de Barros Dantas; Marcelo Mendes Brandão

doi:10.1007/978-3-030-80352-0_6

Interactomes: Experimental and In Silico Approaches

Adv Exp Med Biol. 2021:1346:107-117. doi: 10.1007/978-3-030-80352-0_6.

Authors

Luíza Lane de Barros Dantas¹, Marcelo Mendes Brandão²

Affiliations

¹ John Innes Centre, Norwich Research Park, Norwich, UK. luiza.bd@gmail.com.
² Center for Molecular Biology and Genetic Engineering, State University of Campinas, Campinas, SP, Brazil.

PMID: 35113398
DOI: 10.1007/978-3-030-80352-0_6

Abstract

Any part of the Central Dogma of Molecular Biology (DNA replication, transcription, and translation) is based on an intricate protein-protein interaction. On this chapter, we will navigate over the techniques that enable us to construct or fulfill the gaps on an interactome study, directly using assessment of the biochemical and/or molecular machinery that allow two proteins to interact with each other; or rely on computational biology techniques to gather information on PPI from public available databases and evaluate this interaction.

Keywords: Bioinformatics; Databases; Interactome; Protein–Protein interactions; Proteome.

MeSH terms

Computational Biology
Databases, Protein
Protein Interaction Mapping*
Proteome*

Substances

Proteome