Association Mapping for Yield Attributing Traits and Yellow Mosaic Disease Resistance in Mung Bean [ Vigna radiata (L.) Wilczek]

Versha Rohilla; Rajesh Kumar Yadav; Atman Poonia; Ravika Sheoran; Gita Kumari; P S Shanmugavadivel; Aditya Pratap

doi:10.3389/fpls.2021.749439

Association Mapping for Yield Attributing Traits and Yellow Mosaic Disease Resistance in Mung Bean [ Vigna radiata (L.) Wilczek]

Front Plant Sci. 2022 Jan 17:12:749439. doi: 10.3389/fpls.2021.749439. eCollection 2021.

Authors

Versha Rohilla¹, Rajesh Kumar Yadav¹, Atman Poonia¹, Ravika Sheoran¹, Gita Kumari², P S Shanmugavadivel², Aditya Pratap²

Affiliations

¹ Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India.
² ICAR-Indian Institute of Pulses Research, Kanpur, India.

Abstract

Mung bean [Vigna radiata (L.) Wilczek] is an important short-duration grain legume widely known for its nutritional, soil ameliorative, and cropping system intensification properties. This study aims at evaluating genetic diversity among mung bean genotypes and detecting genomic regions associated with various yield attributing traits and yellow mosaic disease (YMD) resistance by association mapping. A panel of 80 cultivars and advanced breeding lines was evaluated for 10 yield-related and YMD resistance traits during kharif (monsoon) and summer seasons of 2018-2019 and 2019-2020. A total of 164 genome-wide simple sequence repeat (SSR) markers were initially screened, out of which 89 were found polymorphic which generated 317 polymorphic alleles with an average of 3.56 alleles per SSR locus. The number of alleles at each locus varied from 2 to 7. The population genetic structure analysis grouped different genotypes in three major clusters and three genetically distinct subpopulations (SPs) (i.e., SP-1, SP-2, and SP-3) with one admixture subpopulation (SP-4). Both cluster and population genetic structure analysis categorized the advanced mung bean genotypes in a single group/SP and the released varieties in other groups/SPs, suggesting that the studied genotypes may have common ancestral history at some level. The population genetic structure was also in agreement with the genetic diversity analysis. The estimate of the average degree of linkage disequilibrium (LD) present at the genome level in 80 mung bean genotypes unveiled significant LD blocks. Over the four seasons, 10 marker-trait associations were observed significant for YMD and four seed yield (SY)-related traits viz., days to flowering, days to maturity, plant height, and number of pods per plant using the mixed linear model (MLM) method. These associations may be useful for marker-assisted mung bean yield improvement programs and YMD resistance.

Keywords: MLM; SSR; Vigna radiata; association mapping; linkage disequilibrium; yield attributing traits.