A clearing-free protocol for imaging intact whole adipose tissue innervation in mice

Jake W Willows; Magdalena Blaszkiewicz; Kristy L Townsend

doi:10.1016/j.xpro.2021.101109

A clearing-free protocol for imaging intact whole adipose tissue innervation in mice

STAR Protoc. 2022 Jan 19;3(1):101109. doi: 10.1016/j.xpro.2021.101109. eCollection 2022 Mar 18.

Authors

Jake W Willows¹, Magdalena Blaszkiewicz¹, Kristy L Townsend¹

Affiliation

¹ Department of Neurological Surgery, The Ohio State University, Columbus, OH 43210, USA.

Abstract

Here we provide a clearing-free protocol for processing intact, whole mount subcutaneous white adipose tissue (scWAT) for immunofluorescence as an alternative to current clearing-based approaches. We use a combination of Z-depth reduction and autofluorescence quenching techniques to fluorescently label, image, and quantify adipose tissue innervation effectively throughout intact mouse tissues without the need for optical clearing or light sheet microscopy. This protocol has been optimized and validated for adipose neurovascular labeling. For complete details on the use and execution of this protocol, please refer to Willows et al. (2021).

Keywords: Metabolism; Microscopy; Model Organisms; Neuroscience.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue* / diagnostic imaging
Animals
Fluorescent Antibody Technique
Imaging, Three-Dimensional* / methods
Mice
Microscopy
Staining and Labeling

Abstract

Publication types

MeSH terms

Grants and funding