Oxygen Ion Implantation Improving Cell Adhesion on Titanium Surfaces through Increased Attraction of Fibronectin PHSRN Domain

Miusi Shi; Wenting Mo; Haoning Qi; Yueqi Ni; Rui Wang; Kailun Shen; Fanyu Zhang; Shuting Jiang; Xuehui Zhang; Lili Chen; Yufeng Zhang; Xuliang Deng

doi:10.1002/adhm.202101983

Oxygen Ion Implantation Improving Cell Adhesion on Titanium Surfaces through Increased Attraction of Fibronectin PHSRN Domain

Adv Healthc Mater. 2022 May;11(10):e2101983. doi: 10.1002/adhm.202101983. Epub 2022 Feb 18.

Authors

Miusi Shi¹, Wenting Mo¹, Haoning Qi¹, Yueqi Ni¹, Rui Wang¹, Kailun Shen¹, Fanyu Zhang¹, Shuting Jiang¹, Xuehui Zhang^{2

3}, Lili Chen^{4

5

6}, Yufeng Zhang¹, Xuliang Deng^{2

7}

Affiliations

¹ The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine, Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, P. R. China.
² National Engineering Laboratory for Digital and Material Technology of Stomatology, NMPA Key Laboratory for Dental Materials, Beijing Laboratory of Biomedical Materials & Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China.
³ Department of Dental Materials & Dental Medical Devices Testing Center, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China.
⁴ Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, P. R. China.
⁵ School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, P. R. China.
⁶ Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, 430022, P. R. China.
⁷ Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China.

PMID: 35104391
DOI: 10.1002/adhm.202101983

Abstract

Mechanistic understanding of fibronectin (FN) adsorption which determines cell adhesion on cell-implant interfaces is significant for improving the osteoconduction and soft-tissue healing of implants. Here, it is shown that the adsorption behavior of FN on the titanium oxide surface (TiO₂ ) is highly relative to its Pro-His-Ser-Arg-Asn (PHSRN) peptide. FN lacking PHSRN fails to bind to surfaces, resulting in inhibited cell adhesion and spreading. Molecular dynamics simulation shows higher affinity and greater adsorption energy of PHSRN peptide with TiO₂ surface due to the stronger hydrogen bonds formed by the serine and arginine residues with O ion of the substrate. Finally, by increasing O content in TiO₂ surfaces through O ion-beam implantation, improving the cell adhesion, cell differentiation, and the subsequent biomineralization on titanium implant is realized. This study reveals the vital role of PHSRN in FN-mediated cell adhesion on implant surfaces, providing a promising new target for further tissue integration and implant success.

Keywords: PHSRN; cell adhesion; fibronectin; ion implantation; titanium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Adhesion
Fibronectins* / chemistry
Oxygen
Peptides / chemistry
Surface Properties
Titanium* / chemistry
Titanium* / pharmacology

Substances

Fibronectins
Peptides
Titanium
Oxygen