TdfH selectively binds metal-loaded tetrameric calprotectin for zinc import

Aloke K Bera; Runrun Wu; Simone Harrison; Cynthia Nau Cornelissen; Walter J Chazin; Nicholas Noinaj

doi:10.1038/s42003-022-03039-y

TdfH selectively binds metal-loaded tetrameric calprotectin for zinc import

Commun Biol. 2022 Jan 31;5(1):103. doi: 10.1038/s42003-022-03039-y.

Authors

Aloke K Bera¹, Runrun Wu², Simone Harrison³, Cynthia Nau Cornelissen⁴, Walter J Chazin³, Nicholas Noinaj^{5

6}

Affiliations

¹ Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA.
² Interdisciplinary Life Science - PULSe, Purdue University, West Lafayette, IN, 47907, USA.
³ Departments of Biochemistry and Chemistry, and Center for Structural Biology, Vanderbilt University, Nashville, TN, 37240, USA.
⁴ Center for Translational Immunology, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, 30303, USA.
⁵ Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA. nnoinaj@purdue.edu.
⁶ Purdue Institute for Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, IN, 47907, USA. nnoinaj@purdue.edu.

Abstract

To combat nutritional immunity, N. gonorrhoeae has evolved systems to hijack zinc and other metals directly from host metal-binding proteins such as calprotectin (CP). Here, we report the 6.1 Å cryoEM structure of the gonococcal surface receptor TdfH in complex with a zinc-bound CP tetramer. We further show that TdfH can also interact with CP in the presence of copper and manganese, but not with cobalt.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Bacterial Outer Membrane Proteins / metabolism*
Biological Transport
Cryoelectron Microscopy
Gene Expression Regulation, Bacterial
Leukocyte L1 Antigen Complex / chemistry*
Models, Molecular
Neisseria gonorrhoeae / metabolism*
Protein Conformation
Zinc / metabolism*

Substances

Bacterial Outer Membrane Proteins
Leukocyte L1 Antigen Complex
TdfH protein, Neisseria
Zinc

Abstract

Publication types

MeSH terms

Substances

Grants and funding