A label-free electrochemical immunosensor for CA125 detection based on CMK-3(Au/Fc@MgAl-LDH)n multilayer nanocomposites modification

Mengdie Wu; Simin Liu; Feifan Qi; Ren Qiu; Jing Feng; Xinshui Ren; Shengzhong Rong; Hongkun Ma; Dong Chang; Hongzhi Pan

doi:10.1016/j.talanta.2022.123254

A label-free electrochemical immunosensor for CA125 detection based on CMK-3(Au/Fc@MgAl-LDH)_n multilayer nanocomposites modification

Talanta. 2022 May 1:241:123254. doi: 10.1016/j.talanta.2022.123254. Epub 2022 Jan 29.

Authors

Mengdie Wu¹, Simin Liu², Feifan Qi², Ren Qiu², Jing Feng³, Xinshui Ren¹, Shengzhong Rong⁴, Hongkun Ma⁴, Dong Chang⁵, Hongzhi Pan⁶

Affiliations

¹ Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; Shanghai University of Medicine and Health Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai, 200093, China.
² Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
³ The College of Medical Technology, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China.
⁴ Public Health School, Mudanjiang Medical University, Mudanjiang, 157011, China.
⁵ Department of Clinical Laboratory, The Affiliated Pudong Hospital, Fudan University, Shanghai, 201399, China. Electronic address: dongchang1969@163.com.
⁶ Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China. Electronic address: panhongzhilaoshi@163.com.

PMID: 35101834
DOI: 10.1016/j.talanta.2022.123254

Abstract

A label-free electrochemical immunosensor was constructed for cancer antigen 125 (CA125) detection based on multiple-enlargement means of layer-by-layer (LBL) assembly of ordered mesoporous carbon (CMK-3), gold nanoparticles (Au NPs) and MgAl layered double hydroxides containing ferrocenecarboxylic acid (Fc@MgAl-LDH). A CMK-3(Au/Fc@MgAl-LDH)n multilaminate nanocomposites was designed using technology of LBL self-assembly among negatively charged Au NPs, positively charged CMK-3 and Fc@MgAl-LDH nanosheets. The CMK-3(Au/Fc@MgAl-LDH)n multilaminate nanocomposites was used as carriers to increase the immobilization of antibody and the number of loading Fc, conductors to strengthen conductivity and enhancers to amplify signal of Fc step-by-step. Besides, this special and excellent way of LBL assembly can immensely amplify the signal of immunosensor and more immobilize the biomolecules, and label-free method is a more simple the measuring way and the procedure. The immunosensor displayed a wider linear range of 0.01 U ml^-1-1000 U ml^-1 and a lower detection limit of 0.004 U ml^-1. Therefore, the sensor can stablely and accurately be applied for CA125 detection in clinical cancer diagnosis.

Keywords: Cancer antigen 125; Electrochemical immunosensor; Ferrocenecarboxylic acid; Layer-by-layer assembly; Layered double hydroxides; Ordered mesoporous carbon.

MeSH terms

Biosensing Techniques* / methods
CA-125 Antigen
Electrochemical Techniques
Gold / chemistry
Graphite* / chemistry
Immunoassay / methods
Limit of Detection
Metal Nanoparticles* / chemistry
Nanocomposites* / chemistry

Substances

CA-125 Antigen
Gold
Graphite