The efficiency of intrauterine infusion of platelet-rich plasma in the treatment of acute endometritis as assessed by endoscopic, Doppler, oxidative, immunohistochemical, and gene expression alterations in jennies

Haithem A Farghali; Naglaa A AbdElKader; Mohamed Fathi; Ibrahim A Emam; Huda O AbuBakr; Samira H Alijuaydi; Noha Y Salem; Marwa S Khattab; Ali Salama; Eman S Ramadan; Shimaa G Yehia; Elshymaa A Abdelnaby

doi:10.1016/j.theriogenology.2022.01.023

The efficiency of intrauterine infusion of platelet-rich plasma in the treatment of acute endometritis as assessed by endoscopic, Doppler, oxidative, immunohistochemical, and gene expression alterations in jennies

Theriogenology. 2022 Mar 15:181:147-160. doi: 10.1016/j.theriogenology.2022.01.023. Epub 2022 Jan 24.

Affiliations

¹ Department of Surgery and Anesthesia, Faculty of Veterinary Medicine, Cairo University, Egypt.
² Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Egypt.
³ Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Egypt.
⁴ Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Egypt.
⁵ Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Egypt.
⁶ Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Egypt. Electronic address: Elshymaa.ahmed@cu.edu.eg.

PMID: 35101679
DOI: 10.1016/j.theriogenology.2022.01.023

Abstract

This study used autologous platelet-rich plasma (PRP) to treat acute endometritis in jennies with follow-up for alterations in uterine hemodynamics, endoscopic, immunohistochemistry, oxidant/antioxidant imbalance, pro-inflammatory regulatory molecules, and transmembrane mucin expressions. Ten jennies suffering from endometritis (acute type; n = 10) were included in the study. PRP was prepared from each animal and two intrauterine infusions one week apart were administrated. Examination and follow-up were done physically, ultrasonographically, endoscopically and samples were taken for histopathology, immunohistochemistry, and bacteriological examination. Blood and uterine fluid samples were taken to estimate biochemical and oxidative stress alterations. Expression of TRAF6 and MUC1 genes was investigated in uterine fluid, at days -1 (day of diagnosis establishment), 7, 14, and 21. Uterine bacteriological examination showed a decrease in bacterial isolates after PRP treatment. The uterine thickness and uterine vascular perfusion as illustrated by color Doppler ultrasonography were significantly decreased in jennies treated by PRP. Uterine spectral wave pattern showed a significant linear increase in pulsatility index only. Three weeks after first PRP treatment, white light endoscopic examination revealed normal uterine body mucosa and uterine horn folds. A high nuclear factor (NF-κB) expression was seen in the mononuclear cells. A significant reduction in oxidative stress biomarkers in both serum and uterine fluid was recorded after PRP treatment. The TRAF-1 gene expression significantly decreased gradually after intrauterine PRP infusion. The MUC-1 gene expression significantly decreased gradually after intrauterine PRP infusion. Both genes were within normal levels by week 3. Endometritis in jennies is associated with an oxidative process, alterations in serum biochemical parameters, Doppler indices, endoscopic appearance, high NF-κB expression, and upregulation of TRAF-1 and MUC-1 expressions. Two intrauterine infusions of autologous PRP restored normal endometrial appearance after acute endometritis.

Keywords: Doppler; Endometritis; Gene expression; Jennies; NF-κB expression; Platelet-rich plasma.

MeSH terms

Animals
Endometritis* / therapy
Endometritis* / veterinary
Equidae
Female
Gene Expression
Oxidative Stress
Platelet-Rich Plasma*