Synthesis of Phthaladyn-29 and Naphthalimide-10, GTP Site Directed Dynamin GTPase Inhibitors

Cecilia C Russell; Kate L Prichard; Nicholas S O'Brien; Adam McCluskey; Phillip J Robinson; Jennifer R Baker

doi:10.1007/978-1-0716-1916-2_18

Synthesis of Phthaladyn-29 and Naphthalimide-10, GTP Site Directed Dynamin GTPase Inhibitors

Methods Mol Biol. 2022:2417:239-258. doi: 10.1007/978-1-0716-1916-2_18.

Authors

Cecilia C Russell¹, Kate L Prichard¹, Nicholas S O'Brien¹, Adam McCluskey¹, Phillip J Robinson², Jennifer R Baker³

Affiliations

¹ Chemistry, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia.
² Cell Signaling Unit, Children's Medical Research Institute, The University of Sydney, Sydney, NSW, Australia.
³ Chemistry, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia. Jennifer.R.Baker@newcastle.edu.au.

PMID: 35099804
DOI: 10.1007/978-1-0716-1916-2_18

Abstract

Herein we describe the detailed synthesis of the dynamin inhibitors Phthaladyn-29 and Napthaladyn-10, and their chemical scaffold matched partner inactive compounds. Combined with the assay data provided, this allows the interrogation of dynamin in vitro and potentially in vivo.

Keywords: Chemical probe; Chemical synthesis; Dynamin inhibitors; Endocytosis; Naphthalimide; Phthaladyn.

MeSH terms

Dynamins / metabolism
Endocytosis*
Guanosine Triphosphate / metabolism
Naphthalimides*

Substances

Naphthalimides
Guanosine Triphosphate
Dynamins