CD133, a Progenitor Cell Marker, is Reduced in Nasal Polyposis and Showed Significant Correlations with TGF-β1 and IL-8

Wagner Vargas Souza Lino; André Luis Lacerda Bachi; José Arruda Mendes Neto; Gabriel Caetani; Jônatas Bussador do Amaral; Rogério Pezato

doi:10.1055/s-0041-1726043

CD133, a Progenitor Cell Marker, is Reduced in Nasal Polyposis and Showed Significant Correlations with TGF-β1 and IL-8

Int Arch Otorhinolaryngol. 2021 Jun 25;26(1):e091-e096. doi: 10.1055/s-0041-1726043. eCollection 2022 Jan.

Authors

Wagner Vargas Souza Lino¹, André Luis Lacerda Bachi^{1

2

3}, José Arruda Mendes Neto¹, Gabriel Caetani⁴, Jônatas Bussador do Amaral¹, Rogério Pezato^{1

4}

Affiliations

¹ Department of Otorhinolaryngology - Head and Neck Surgery, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
² Instituto Brasileiro Ensino/Pesquisa em Imunologia Pulmonar, São Paulo, SP, Brazil.
³ Postgraduation Program in Health Science, Universidade Santo Amaro), Sao Paulo, SP, Brazil.
⁴ Hospital Universitário Gafree e Guinle, Universidade Federal do Estado do Rio de Janeiro,, Rio de Janeiro, RJ, Brazil.

Abstract

Introduction Combination of chronic inflammation and an altered tissue remodeling process are involved in the development of Chronic Rhinosinusitis with Nasal Polyps (CRSwNP). Studies demonstrated that mesenchymal stem cells expressing the progenitor gene CD133 were involved in a significant reduction of the chronic inflammatory process in the polypoid tissue. Objective To evaluate the levels of CD133 (Prominin-1) in nasal polypoid tissue and its correlation with interleukin-8 (IL-8) and transforming growth factor β1 (TGF-β1). Methods A total of 74 subjects were divided in the following groups: control group ( n = 35); chronic rhinosinusitis with nasal polyps nonpresenting comorbid asthma and aspirin intolerance (CRSwNPnonAI) group ( n = 27); and chronic rhinosinusitis with nasal polyps presenting comorbid asthma and aspirin intolerance (CRSwNPAI) group ( n = 12). Histologic analysis and also evaluation of the concentration of CD133, IL-8, and TGF-β1 by enzyme-linked immunosorbent assay (ELISA) kits were performed in nasal tissue obtained from nasal polypectomy or from middle turbinate tissue. Results Higher eosinophilic infiltration was found in both CRSwNP groups by histologic analysis. Lower levels of TGF-β1 and IL-8 were observed in both CRSwNP groups when compared with the control group, whereas the CD133 levels were significantly reduced only in the CRSwNPnonAI group compared with the control group. Conclusion It was demonstrated that the nasal mucosa presenting polyposis showed a significant reduction of CD133 levels, and also that this reduction was significantly correlated with the reduction of TGF-β1 levels, but not with IL-8 levels. Therefore, these findings may be involved in the altered inflammatory and remodeling processes observed in the nasal polyposis.

Keywords: AC133 antigen; aspirin-induced asthma; interleukin-8; nasal polyps; nose diseases; transforming growth factor β1.

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