Background: Disruption of ICT1 has been known to cause a significant deficiency in the phospholipid composition which is necessary for cell stress adaptation. However, the effects of ICT1 deletion on antioxidant research are not clear.
Objective: Construct a knockout strain to investigate the efficacy of ICT1 on antioxidant activity.
Methods: The antioxidant-related genes and phospholipid-related genes were determined by RT-PCR, the cell wall shape was observed by TME, and CWI pathway phosphorylation experiments were also analyzed by HPLC.
Results: The expression of antioxidant related genes and phospholipid-related genes has a slight reduction compared to the wild type. The cell wall was observed impaired with apparent CWI pathway phosphorylation weakening in the mutant.
Conclusion: These findings indicate the role of ICT1 on antioxidant activity because it not only directly affects phospholipid composition but also further leads to the activation of CWI.
Keywords: CWI; ICT1; Oxidative stress; Plasma membrane; Saccharomyces cerevisiae.
© 2021. The Author(s) under exclusive licence to The Genetics Society of Korea.