Generating 3D human cardiac constructs from pluripotent stem cells

Chang Liu; Xing Feng; Guoping Li; Priyanka Gokulnath; Junjie Xiao

doi:10.1016/j.ebiom.2022.103813

Generating 3D human cardiac constructs from pluripotent stem cells

EBioMedicine. 2022 Feb:76:103813. doi: 10.1016/j.ebiom.2022.103813. Epub 2022 Jan 27.

Authors

Chang Liu¹, Xing Feng¹, Guoping Li², Priyanka Gokulnath², Junjie Xiao³

Affiliations

¹ Institute of Geriatrics (Shanghai University), Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Nantong 226011, China; Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, Shanghai Engineering Research Center of Organ Repair, School of Life Science, Shanghai University, Shanghai 200444, China.
² Cardiovascular Division of the Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
³ Institute of Geriatrics (Shanghai University), Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Nantong 226011, China; Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, Shanghai Engineering Research Center of Organ Repair, School of Life Science, Shanghai University, Shanghai 200444, China. Electronic address: junjiexiao@shu.edu.cn.

Abstract

Human pluripotent stem cell (hPSC) technology has offered nearly infinite opportunities to model all kinds of human diseases in vitro. Cardiomyocytes derived from hPSCs have proved to be efficient tools for cardiac disease modeling, drug screening and pathological mechanism studies. In this review, we discuss the advantages and limitations of 2D hPSC-cardiomyocyte (hPSC-CM) system, and introduce the recent development of three-dimensional (3D) culture platforms derived from hPSCs. Although the development of bioengineering technologies has greatly improved 3D platform construction, there are certainly challenges and room for development for further in-depth research.

Keywords: Cardiac 3D constructs; Cardiac organoid; Engineered heart tissue; Heart-on-chip; Microtissue; Pluripotent stem cells; Spheroids.

Publication types

Review

MeSH terms

Cell Differentiation / genetics
Humans
Myocytes, Cardiac
Pluripotent Stem Cells*