Species-specific variation of photosynthesis and mesophyll conductance to ozone and drought in three Mediterranean oaks

Abstract

Mesophyll conductance (g_mCO2 ) is one of the most important components in plant photosynthesis. Tropospheric ozone (O₃ ) and drought impair physiological processes, causing damage to photosynthetic systems. However, the combined effects of O₃ and drought on g_mCO2 are still largely unclear. We investigated leaf gas exchange during mid-summer in three Mediterranean oaks exposed to O₃ (ambient [35.2 nmol mol^-1 as daily mean]; 1.4 × ambient) and water treatments (WW [well-watered] and WD [water-deficit]). We also examined if leaf traits (leaf mass per area [LMA], foliar abscisic acid concentration [ABA]) could influence the diffusion of CO₂ inside a leaf. The combination of O₃ and WD significantly decreased net photosynthetic rate (P_N ) regardless of the species. The reduction of photosynthesis was associated with a decrease in g_mCO2 and stomatal conductance (g_sCO2 ) in evergreen Quercus ilex, while the two deciduous oaks (Q. pubescens, Q. robur) also showed a reduction of the maximum rate of carboxylation (V_cmax ) and maximum electron transport rate (J_max ) with decreased diffusive conductance parameters. The reduction of g_mCO2 was correlated with increased [ABA] in the three oaks, whereas there was a negative correlation between g_mCO2 with LMA in Q. pubescens. Interestingly, two deciduous oaks showed a weak or no significant correlation between g_sCO2 and ABA under high O₃ and WD due to impaired stomatal physiological behaviour, indicating that the reduction of P_N was related to g_mCO2 rather than g_sCO2 . The results suggest that g_mCO2 plays an important role in plant carbon gain under concurrent increases in the severity of drought and O₃ pollution.