Remediation of Pb-diesel fuel co-contaminated soil using nano/bio process: subsequent use of nanoscale zero-valent iron and bioremediation approaches

Abstract

The effectiveness of the nano/bio process was investigated as a remediation option for co-contaminated soils. Nano/bio process is a hybrid treatment method that may be defined as the use of nanoscale zero-valent iron (nZVI) and bioremediation approaches subsequently/concurrently. Different bioremediation approaches (bioattenuation, biostimulation, and/or bioaugmentation) were performed together with nZVI application to remediate Pb- and diesel fuel-spiked soils. Nutrient (N and P) and activated sludge amendment were made to realize biostimulation and bioaugmentation, respectively. The nZVI application decreased the total percentage of the most mobile and bioavailable soil Pb fractions (exchangeable and carbonate-bound) from 68.3 to 31.7%. The biodegradation levels of nZVI-applied co-contaminated soils were significantly higher than the soils without nZVI indicating the positive effect of the reduced mobility, bioavailability, and toxicity of Pb content. The use of nano/biostimulation or nano/bioaugmentation treatments resulted in higher than 60% total n-alkane degradation, whereas 89.5% degradation was obtained by using nano/biostimulation + bioaugmentation. Hydrocarbon-degrader strains belonging to phyla Actinobacteria, Proteobacteria, or Firmicutes were identified from samples subjected to nano/bio process and the strains from biostimulation and bioaugmentation treatments were different. These results indicate that the stress on the microbial population caused by the co-contamination might be subsided and the biodegradation of alkanes might be improved by using the nano/bio process.

Keywords: Bioaugmentation; Biodegradation; Biostimulation; Co-contamination; Immobilization; nZVI.