m6Adecom: Analysis of m6A profile matrix based on graph regularized non-negative matrix factorization

Abstract

Epitranscriptomic m⁶A methylation is shown to mediate extensive regulations under the context of various RNA binding protein (RBP) readers. With m⁶A methylation data has reached a sizable scale, the functional context-aware analysis of m⁶A profiles is becoming more feasible and demanded. In this study, we employed graph regularized non-negative matrix factorization (GNMF) for m⁶A profile analysis and comparison, where the RBP binding preference of m⁶A sites were incorporated as the functional context-based graph constraint term. Compared to the baseline non-negative matrix factorization (NMF) method, this GNMF-based method could better capture the distinctions in multiple functional characteristics between different group of m⁶A sites, including but not limited to the associated biological pathways and disease genes. We further established m6Adecom, an online tool that can be used for correlation and enrichment analysis of m⁶A profiles using the matrix decomposition result from GNMF, and gene set enrichment analysis based on the high-score m⁶A sites. m6Adecom is freely accessible at http://www.rnanut.net/m6adecom.

Keywords: Epitranscriptomic modification; Graph regularized non-negative matrix factorization; RNA binding protein; Webserver; m6A methylation.