Evaluation of liver T1rho and T2 values in acute liver inflammation models using 7T-MRI

Narumi Arihara; Shigeyoshi Saito; Reika Sawaya; Ryutarou Onishi; Keiho Tsuji; Akiko Ohki; Junpei Ueda; Daisuke Morimoto-Ishiwaka

doi:10.1016/j.mri.2022.01.010

Evaluation of liver T_1rho and T₂ values in acute liver inflammation models using 7T-MRI

Magn Reson Imaging. 2022 May:88:20-24. doi: 10.1016/j.mri.2022.01.010. Epub 2022 Jan 25.

Authors

Narumi Arihara¹, Shigeyoshi Saito², Reika Sawaya¹, Ryutarou Onishi¹, Keiho Tsuji¹, Akiko Ohki³, Junpei Ueda¹, Daisuke Morimoto-Ishiwaka⁴

Affiliations

¹ Department of Medical Physics and Engineering, Division of Health Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
² Department of Medical Physics and Engineering, Division of Health Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, Japan; Department of Advanced Medical Technologies, National Cardiovascular and Cerebral Research Center, Suita, Osaka, Japan. Electronic address: saito@sahs.med.osaka-u.ac.jp.
³ Department of Medical Physics and Engineering, Division of Health Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, Japan; Department of Medical Technology, Kyoto University Hospital, Kyoto, Kyoto, Japan.
⁴ Department of Medical Physics and Engineering, Division of Health Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, Japan; Department of Radiology, Kindai University, Osaka-Sayama, Osaka, Japan.

PMID: 35091025
DOI: 10.1016/j.mri.2022.01.010

Abstract

Purpose: We measured the T_1rho and T₂ values the liver of acute liver inflammation model mice administered carbon tetrachloride (CCl₄) after 3 days and 6 days after dispensed, and we compared and examined whether each relaxation time can be used for detect acute liver inflammation.

Methods: To create an acute liver inflammation model, a mixture of 0.2 ml / 100 g of CCl₄ with an equal amount of Sesame Oil was administered once intraperitoneally to C57BL / 6JJmsSlc mice (n = 15). On the 3 days and 6 days after administration, we acquired T_1rho mapping images and T₂ mapping images of the liver under respiratory synchronization using for preclinical 7T-MRI, and we measured T_1rho and T₂ values and compared statistically.

Results: The liver T_1rho value of control mice was 33.9 ± 2.5 ms before CCl₄ administration, 43.2 ± 4.9 ms (p < 0.01) on the 3 days post CCl₄ injection, and 41.0 ± 1.2 ms (p < 0.001) on the 6 days post CCl₄ injection. The rate showed a significant increase of 27% on the 3 days after, as well as significant increase of 21% on the 6 days after. On the other hand, the liver T₂ value of control mice was 26.7 ± 1.9 ms before CCl₄ administration, 31.5 ± 3.4 ms (p < 0.05) 3 days post CCl₄ injection, and 29.0 ± 2.0 ms (p = 0.06) 6 days post CCl₄ injection. The rate 3 days after CCl₄ administration showed a significant increase of 18%, after 6 days rate increased 9%, but no significant difference was confirmed compared with normal mice.

Conclusions: The T_1rho value changed significantly compared to the T₂ value, and a continuous change was observed even after 6 days. T_1rho mapping can diagnose acute liver inflammation.

Keywords: Acute liver inflammation; Mice; T(1rho) value; T(2) value.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carbon Tetrachloride
Inflammation / diagnostic imaging
Liver* / diagnostic imaging
Magnetic Resonance Imaging* / methods
Mice
Mice, Inbred C57BL

Substances

Carbon Tetrachloride