Generation and characterization of human umbilical cord blood-derived induced pluripotent stem cells (KRIBBi005-A)

Hyebin Koh; Xing Zhen; Jieun Kim; Hye-Yeong Ha; Jong-Hee Lee

doi:10.1016/j.scr.2022.102674

Generation and characterization of human umbilical cord blood-derived induced pluripotent stem cells (KRIBBi005-A)

Stem Cell Res. 2022 Apr:60:102674. doi: 10.1016/j.scr.2022.102674. Epub 2022 Jan 19.

Authors

Hyebin Koh¹, Xing Zhen², Jieun Kim³, Hye-Yeong Ha⁴, Jong-Hee Lee⁵

Affiliations

¹ Futuristic Animal Resource & Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Functional Genomics, KRIBB School of Bioscience, University of Science and Technology (UST), Daejeon, Republic of Korea.
² National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Nanoscience and Nanotechnology, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea.
³ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
⁴ Division of Intractable Disease Research, Korea National Institute of Health, Cheongju, Republic of Korea.
⁵ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Functional Genomics, KRIBB School of Bioscience, University of Science and Technology (UST), Daejeon, Republic of Korea. Electronic address: jonglee@kribb.re.kr.

PMID: 35085946
DOI: 10.1016/j.scr.2022.102674

Abstract

Induced pluripotent stem cells (iPSCs) hold great promise as a potential source for regenerative medicine. Umbilical cord blood (UCB) cells possess a high level of stem cells, exhibit less immune rejection, and have fewer DNA mutations and, thus, are an easily accessible and valuable cell source for iPSC generation for basic research and clinical applications. Here, we reprogrammed CD34⁺-UCB cells with the group O/D-negative (RhD^-) blood type as a universal source for red blood cell (RBC) generation. The resulting iPSCs, with features typical of pluripotent stem cells, will further advance the development of various therapeutics, including blood transfusion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, CD34 / metabolism
Fetal Blood / metabolism
Humans
Induced Pluripotent Stem Cells* / metabolism
Pluripotent Stem Cells*
Regenerative Medicine

Substances

Antigens, CD34