Antiosteosarcoma effects of novel 23-nor-3,4-seco-3-acetallupane triterpenoids from Acanthopanax gracilistylus W.W. Smith var. gracilistylus in 143B cells

Abstract

Three unprecedented 23-nor-3,4-seco-3-acetallupane triterpenoids, gracilistylacid A-C (1-3), along with three known lupanoids (4-6), were isolated from the aerial parts of Acanthopanax gracilistylus W.W. Smith var. gracilistylus. Compounds 1-3 may be biosynthetically formed via carboxylation, decarboxylation, cycloreversion, and aldolization reactions based on impressic acid (4). The structures of all compounds were characterized by spectroscopic techniques and X-ray craystallographyic studies. Compounds 3 and 4 exerted anti-osteosarcoma effects through an inhibition of cell migration and vasculogenic mimicry (VM) formation in 143B cells in vitro.

Keywords: 23-nor-3,4-seco-3-Acetallupanoids; Acanthopanax gracilistylus W. W. Smith var. gracilistylus; Migration; VM formation.