The association of IL-3, IL-17A, and IL 27 serum levels with susceptibility to toxoplasmosis in recurrent abortion of Iraqi women

Noor Nihad Baqer; Entsar Jabbar Saheb; Najwa Shihab Ahmed; Najah Abdulnabi Ali Alhadad

doi:10.1016/j.exppara.2022.108217

The association of IL-3, IL-17A, and IL 27 serum levels with susceptibility to toxoplasmosis in recurrent abortion of Iraqi women

Exp Parasitol. 2022 Mar:234:108217. doi: 10.1016/j.exppara.2022.108217. Epub 2022 Jan 25.

Authors

Noor Nihad Baqer¹, Entsar Jabbar Saheb², Najwa Shihab Ahmed³, Najah Abdulnabi Ali Alhadad⁴

Affiliations

¹ Ministry of Science and Technology, Water and Environment Directorate, Iraq. Electronic address: nour.nohad1102a@sc.uobaghdad.edu.iq.
² Department of Biology, Collage of Science /Baghdad University, Iraq.
³ Biotechnology Research Center, Al-Nahrain University, Iraq.
⁴ Department Obstetric and Gynaecology, Al-Alawiya Maternity Teaching Hospital, Baghdad, Iraq.

PMID: 35085575
DOI: 10.1016/j.exppara.2022.108217

Abstract

Cytokines are a group of immunomodulatory proteins leading to a variety of immune reactions in the human; these cytokines play a significant role in the development of appropriate immune responses against T. gondii. This study aims to reveal the association of toxoplasmosis with serum levels of IL-3, IL-17A, and IL-27 in aborted women. The blood samples of patients and controls were collected from Al-Alawiya Maternity Teaching Hospital/Baghdad/Iraq from 2019 to 2020 for detecting anti-T. gondii antibodies (IgG and IgM) and the level of interleukins by ELISA. The results of TORCH by rapid test for recurrent abortion recorded 25.3% seropositive for anti-Toxoplasma antibodies, and 31.5% seropositive for one or more cases of TORCH test (Cytomegalovirus, Rubella, and Herpes). Whereas the results for anti-T. gondii IgG and IgM antibodies were shown elevated positivity percentages by ELISA test; these percentages were 56.2% in recurrent abortion women with significant differences (P < 0.05). The results suggested that the IL-3 serum concentration of pregnant women, recurrent abortion, and recurrent abortion with toxoplasmosis was declined versus healthy women with significant differences (p < 0.05). However, the results revealed that the concentration of IL-17A in recurrent abortion, and recurrent abortion with toxoplasmosis elevated versus healthy women and pregnant women with significant difference (p < 0.05). Whereas the results indicated that the IL-27 serum concentration elevated with significant differences in recurrent abortion with toxoplasmosis group compared to healthy women, pregnant women, and recurrent abortion. Interestingly, the serum levels for IL-27 increased comparing to the levels of IL-3 and IL-17A in all groups with significant differences (P < 0.05). In conclusion, it appeared in this study that the role of IL-3, IL-17A, and IL-27 in the maternal immune response during infections can lead to abortion.

Keywords: IL-17A; IL-27; IL-3; Recurrent abortion women; Toxoplasmosis.

MeSH terms

Abortion, Habitual / immunology
Abortion, Habitual / parasitology*
Adult
Antibodies, Protozoan / blood
Disease Susceptibility
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin G / blood
Immunoglobulin M / blood
Interleukin-17 / blood*
Interleukin-27 / blood*
Interleukin-3 / blood*
Iraq
Pregnancy
Toxoplasmosis / complications
Toxoplasmosis / immunology*

Substances

Antibodies, Protozoan
Immunoglobulin G
Immunoglobulin M
Interleukin-17
Interleukin-27
Interleukin-3