Enhanced mechanical properties and biocompatibility on BC/HAp composite through calcium gluconate fortified bacterial

Lin Shi; Tao Wang; Lei Yang; Chuntao Chen; Ran Dou; Xiaoli Yang; Bianjing Sun; Baojing Zhou; Lei Zhang; Dongping Sun

doi:10.1016/j.carbpol.2021.119085

Enhanced mechanical properties and biocompatibility on BC/HAp composite through calcium gluconate fortified bacterial

Carbohydr Polym. 2022 Apr 1:281:119085. doi: 10.1016/j.carbpol.2021.119085. Epub 2022 Jan 4.

Authors

Lin Shi¹, Tao Wang¹, Lei Yang¹, Chuntao Chen², Ran Dou³, Xiaoli Yang¹, Bianjing Sun¹, Baojing Zhou³, Lei Zhang⁴, Dongping Sun⁵

Affiliations

¹ Institute of Chemicobiology and Functional Materials, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, 200 Xiao Ling Wei, Nanjing 210094, China.
² Institute of Chemicobiology and Functional Materials, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, 200 Xiao Ling Wei, Nanjing 210094, China. Electronic address: chchunt@njust.edu.cn.
³ Computational Institute for Molecules and Materials, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, 200 Xiao Ling Wei, Nanjing 210094, China.
⁴ Institute of Chemicobiology and Functional Materials, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, 200 Xiao Ling Wei, Nanjing 210094, China. Electronic address: leizhang@njust.edu.cn.
⁵ Institute of Chemicobiology and Functional Materials, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, 200 Xiao Ling Wei, Nanjing 210094, China. Electronic address: hysdp@njust.edu.cn.

PMID: 35074101
DOI: 10.1016/j.carbpol.2021.119085

Abstract

Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering. The conventional biomimetic mineralization method takes a long time with unsatisfactory mechanical properties and biocompatibility. Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid. Results show spherical porous HAp in the size of 100-200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization. Molecular dynamics simulation shows that the aggregation of cellulose units in aqueous solution can enhance the adsorption of calcium ions. By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering.

Keywords: Bacterial cellulose; Biomineralization; Calcium gluconate; Hydroxyapatite; In situ biosynthesis.

MeSH terms

Bacteria
Calcium Gluconate / pharmacology
Cellulose* / chemistry
Durapatite* / chemistry
Tissue Engineering / methods

Substances

Cellulose
Durapatite
Calcium Gluconate