AlliGator: A Phasor Computational Platform for Fast in vivo Lifetime Analysis

Sez-Jade Chen; Nattawut Sinsuebphon; Margarida Barroso; Xavier Intes; Xavier Michalet

doi:10.1364/omp.2017.omtu2d.2

AlliGator: A Phasor Computational Platform for Fast in vivo Lifetime Analysis

Opt Mol Probes Imaging Drug Deliv. 2017 Apr:2017:OmTu2D.2. doi: 10.1364/omp.2017.omtu2d.2.

Authors

Sez-Jade Chen¹, Nattawut Sinsuebphon¹, Margarida Barroso², Xavier Intes¹, Xavier Michalet³

Affiliations

¹ Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
² Center for Cardiovascular Sciences, Albany Medical College, Albany, NY, 12208, USA.
³ Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, 90095, USA.

Abstract

A fast time-gated phasor analysis tool was developed and used to monitor kinetics of NIR FLIM-FRET in vitro and in vivo. The results were validated by comparison with standard two-component fluorescence decay fitting analysis.

Keywords: (170.3650) Lifetime-based sensing; (170.6920) Time-resolved imaging; (260.2510) Fluorescence.

Grants and funding

R01 EB019443/EB/NIBIB NIH HHS/United States