Building invitro 3D human multicellular models of high-grade serous ovarian cancer

STAR Protoc. 2022 Jan 11;3(1):101086. doi: 10.1016/j.xpro.2021.101086. eCollection 2022 Mar 18.

Abstract

Three-dimensional (3D), multicellular invitro models provide a useful platform for studying human cancer biology, particularly through deconvolution of the tumor microenvironment, or where animal models do not recapitulate the human condition. Here, we detail a protocol for building human multicellular models made of patient-derived primary cells and malignant cell lines, which recapitulate features of the tumor microenvironment. This protocol is optimized for building 3D models of high-grade serous ovarian cancer omental metastasis but can be adapted for modeling other cancers. For complete details on the use and execution of this profile, please refer to Delaine-Smith et al. (2021) and Malacrida et al. (2021).

Keywords: Cancer; Cell Biology; Cell culture; Cell isolation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Female
  • Humans
  • Ovarian Neoplasms* / metabolism
  • Peritoneal Neoplasms*
  • Tumor Microenvironment