Characterization and phylogenetic analysis of the mitochondrial genome of Holotrichia parallela (Coleoptera: Scarabaeidae: Melolonthinae)

Jing Zhao; Feng Zhang; Jingjing Yuan; Zhenjie Du; Xuebin Qi

doi:10.1080/23802359.2021.2016082

Characterization and phylogenetic analysis of the mitochondrial genome of Holotrichia parallela (Coleoptera: Scarabaeidae: Melolonthinae)

Mitochondrial DNA B Resour. 2022 Jan 18;7(1):208-210. doi: 10.1080/23802359.2021.2016082. eCollection 2022.

Authors

Jing Zhao^{1

2

3}, Feng Zhang⁴, Jingjing Yuan⁵, Zhenjie Du^{2

3}, Xuebin Qi^{2

3}

Affiliations

¹ College of Materials Science and Engineering, Henan Institute of Technology, Xinxiang, China.
² Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang, China.
³ Key Laboratory of High-efficient and Safe Utilization of Agriculture Water Resources of CAAS, Xinxiang, China.
⁴ College of Intelligent Engineering, Henan Institute of Technology, Xinxiang, China.
⁵ Henan Institute of Metrology, China Henan Institute of Metrology, Xinxiang, China.

Abstract

Holotrichia parallela (Motschulsky, 1854) is an important pest for peanut, potato, and soybean in China, and it causes great economic losses. In this study, we sequenced and analyzed the complete mitochondrial genome (mitogenome) of H. parallela. This mitogenome was 16,975 bp long and encoded 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), and two ribosomal RNA genes (rRNAs). Gene order was conserved and identical to most other previously sequenced Scarabaeidae. Most PCGs of H. parallela have the conventional start codons ATN, with the exception of cox1 (AAC). Except for three genes (cox1, cox2, and cox3) end with the incomplete stop codon T-, all other PCGs terminated with the stop codon TAA or TAG. Phylogenetic analysis positioned H. parallela in a well-supported clade with Rhopaea magnicornis, Polyphylla gracilicornis, and Melolontha hippocastani. The relationships (Dynastinae+(Cetoniinae+(Melolonthinae+(Rutelinae + Scarabaeinae)))) were supported in Scarabaeidae.

Keywords: Holotrichia parallela; Scarabaeidae; mitochondrial genome; phylogenetic analysis.

Grants and funding

This study was supported by National Natural Science Foundation of China [21906041 and 51779260] and Scientific and Technological Project of Henan Province [192102110051, 192102110198, and 202102110123].