Biotinylated subunit of 3-methylcrotonyl-CoA carboxylase encoding gene (AtMCCA) participating in Arabidopsis resistance to carbonate Stress by transcriptome analysis

Yao Wang; Xiaoxue Ye; Tetsuo Takano; Shenkui Liu; Yuanyuan Bu

doi:10.1016/j.plantsci.2021.111130

Biotinylated subunit of 3-methylcrotonyl-CoA carboxylase encoding gene (AtMCCA) participating in Arabidopsis resistance to carbonate Stress by transcriptome analysis

Plant Sci. 2022 Feb:315:111130. doi: 10.1016/j.plantsci.2021.111130. Epub 2021 Nov 23.

Authors

Yao Wang¹, Xiaoxue Ye¹, Tetsuo Takano², Shenkui Liu³, Yuanyuan Bu⁴

Affiliations

¹ Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin, 150040, China; College of Life Science, Northeast Forestry University, Harbin, 150040, China.
² Asian Natural Environmental Science Center (ANESC), University of Tokyo, Nishitokyo, Tokyo, 188-0002, Japan.
³ Department of Silviculture, State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin'an, Zhejiang, 311300, China. Electronic address: shenkuiliu@nefu.edu.cn.
⁴ Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin, 150040, China; College of Life Science, Northeast Forestry University, Harbin, 150040, China. Electronic address: yuanyuanbu@nefu.edu.cn.

PMID: 35067300
DOI: 10.1016/j.plantsci.2021.111130

Abstract

Soil salinization is a major factor impacting modern agricultural production, and alkaline soils contain large amounts of NaHCO₃. Therefore, understanding plant tolerance to high levels of NaHCO₃ is essential. In this study, a transcriptome analysis of shoot and root tissues of wild-type Arabidopsis thaliana was conducted at 0, 4, 12, 24 and 48 h after exposure to a 3 mM NaHCO₃ stress. We focused on differentially expressed genes (DEGs) in roots identified in the early stages (4 h and 12 h) of the NaHCO₃ stress response that were enriched in GO term, carboxylic acid metabolic process, and utilize HCO₃^-. Six genes were identified that exhibited similar expression patterns in both the RNA-seq and qRT-PCR data. We also characterized the phenotypic response of AtMCCA-overexpressing plants to carbonate stress, and found that the ability of AtMCCA-overexpressing plants to tolerate carbonate stress was enhanced by the addition of biotin to the growth medium.

Keywords: AtMCCA; Biotin; NaHCO(3) stress; Transcriptome.

MeSH terms

Acyl Coenzyme A / genetics*
Acyl Coenzyme A / metabolism*
Adaptation, Physiological / genetics*
Arabidopsis / genetics*
Arabidopsis / growth & development*
Carbamates / adverse effects*
Gene Expression Regulation, Plant
Genes, Plant
Transcriptome*

Substances

Acyl Coenzyme A
Carbamates