Transcriptome and metabolome analyses of the immune response to light stress in the hybrid grouper (Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀)

Z M Zhong; J Zhang; B G Tang; F F Yu; Y S Lu; G Hou; J Y Chen; Z X Du

doi:10.1016/j.animal.2021.100448

Transcriptome and metabolome analyses of the immune response to light stress in the hybrid grouper (Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀)

Animal. 2022 Feb;16(2):100448. doi: 10.1016/j.animal.2021.100448. Epub 2022 Jan 20.

Authors

Z M Zhong¹, J Zhang², B G Tang², F F Yu³, Y S Lu⁴, G Hou¹, J Y Chen¹, Z X Du¹

Affiliations

¹ College of Fishery, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China.
² College of Fishery, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, Guangdong 524006, China.
³ College of Fishery, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, Guangdong 524006, China. Electronic address: yufeifei2000@163.com.
⁴ College of Fishery, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen, Guangdong 518120, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China.

PMID: 35065313
DOI: 10.1016/j.animal.2021.100448

Abstract

Light intensity is an important environmental factor that affects fish growth and health through multiple physiological activities and metabolism and eventually impacts aquaculture harvest. There is a need to evaluate the fish stress response to light intensities, which will benefit aquaculture. Here, hybrid grouper (Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀) was treated with three light intensities for evaluation of the light stress response, including high light intensity (1 250 lx), low light intensity (10 lx) and moderate light intensity (250 lx). Transcriptome analysis showed that a total of 71 318 unigene sequences were obtained with an N50 of 2 589 bp. Compared to the control group (250 lx), 1 697 differentially expressed genes (DEGs), a considerable quantity, were detected in the 1 250 lx group. Among those genes, 548 were upregulated, and the remaining 149 genes showed decreased expression. Comparatively small numbers of DEGs were detected in the 10 lx group; 54 out of 103 genes exhibited upregulated expression, and 49 genes showed downregulation. For further KEGG analysis, 82 DEGs were enriched in nine common signalling pathways in immunity, of which 73 DEGs were significantly inhibited in the 1 250 lx group. In contrast, only 11 DEGs were enriched in three immunity pathways, with nine DEGs showing a significant increase in the 10 lx group. The metabolome analysis revealed 59 and 44 differential metabolites (DMs) from the 1 250 lx and 10 lx groups, respectively. Of note, those DMs from the 1 250 lx-treated group were tendentiously involved in amino acid metabolism and lipid metabolism pathways, while the purine metabolism, amino acid metabolism and lipid metabolism pathways were mostly found in the 10 lx treatment group. In summary, our data indicated that high light intensity significantly inhibited the immune response in hybrid grouper, while low light intensity presented low stimulation of immune activity. In addition, both high and low light intensity could inhibit protein synthesis and amino acid metabolism. Taken together, hybrid grouper exhibited a much milder stress response to low light intensity than to high light intensity.

Keywords: Enrichment analysis; Light intensity; Metabolomic; Stress response; Transcriptomic.

MeSH terms

Animals
Bass* / genetics
Gene Expression Profiling / veterinary
Immunity / genetics
Metabolome
Transcriptome