Transcriptome profiling reveals major structural genes, transcription factors and biosynthetic pathways involved in leaf senescence and nitrogen remobilization in rainfed spring wheat under different nitrogen fertilization rates

Zechariah Effah; Lingling Li; Junhong Xie; Benjamin Karikari; Chang Liu; Aixia Xu; Min Zeng

doi:10.1016/j.ygeno.2022.110271

Transcriptome profiling reveals major structural genes, transcription factors and biosynthetic pathways involved in leaf senescence and nitrogen remobilization in rainfed spring wheat under different nitrogen fertilization rates

Genomics. 2022 Mar;114(2):110271. doi: 10.1016/j.ygeno.2022.110271. Epub 2022 Jan 19.

Authors

Zechariah Effah¹, Lingling Li², Junhong Xie³, Benjamin Karikari⁴, Chang Liu³, Aixia Xu³, Min Zeng³

Affiliations

¹ State Key Laboratory of Arid Land Crop Science, Lanzhou, China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; CSIR-Plant Genetic Resources Research Institute (PGRRI), P. O. Box 7, Bunso, Ghana.
² State Key Laboratory of Arid Land Crop Science, Lanzhou, China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China. Electronic address: lill@gsau.edu.cn.
³ State Key Laboratory of Arid Land Crop Science, Lanzhou, China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
⁴ Department of Crop Science, Faculty of Agriculture, Food and Consumer Sciences, University for Development Studies, P. O Box TL 1882, Tamale, Ghana.

PMID: 35065192
DOI: 10.1016/j.ygeno.2022.110271

Abstract

The present study was undertaken to profile transcriptional changes in flag leaves between anthesis and end of grain filling stages of rainfed spring wheat cultivar under varying nitrogen (N) application rates: 0 kg/ha (NN), 52.5 kg/ha (LN), and 210 kg/ha (HN). A total of 4485 and 4627 differentially expressed genes (DEGs) were detected in LN and HN, respectively. The differential application of N altered several pathways; including plant hormone signal transduction, mitogen-activated protein kinase signaling pathway-plant, photosynthesis, phenylpropanoid biosynthesis and ATP-binding cassette transporters. Jasmonic acid, abscisic acid, salicylic acid and brassinosteroid related genes promoted leaf senescence in NN or LN, whereas auxin, gibberellin acid and cytokinins genes inhibited leaf senescence in HN. Major transcription factors: auxin/indole-3-acetic acid (AUX/IAA), no apical meristem (NAC) and WRKY expressed higher in either HN or LN than NN. The DEGs, pathways and transcription factors provide valuable insight for manipulation of leaf senescence and N remobilization in wheat.

Keywords: Biosynthetic pathway; Differentially expressed genes; Flag leaf; Nitrogen use efficiency; Phytohormones.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosynthetic Pathways
Fertilization
Gene Expression Profiling
Gene Expression Regulation, Plant
Indoleacetic Acids / metabolism
Nitrogen / metabolism
Plant Leaves / genetics
Plant Leaves / metabolism
Plant Senescence
Transcription Factors* / genetics
Transcription Factors* / metabolism
Transcriptome
Triticum* / genetics
Triticum* / metabolism

Substances

Indoleacetic Acids
Transcription Factors
Nitrogen