Immunomodulation effect of polysaccharides from liquid fermentation of Monascus purpureus 40269 via membrane TLR-4 to activate the MAPK and NF-κB signaling pathways

Liuming Xie; Zhibing Huang; Hui Meng; Xiaoyi Shi; Jianhua Xie

doi:10.1016/j.ijbiomac.2022.01.045

Immunomodulation effect of polysaccharides from liquid fermentation of Monascus purpureus 40269 via membrane TLR-4 to activate the MAPK and NF-κB signaling pathways

Int J Biol Macromol. 2022 Mar 15:201:480-491. doi: 10.1016/j.ijbiomac.2022.01.045. Epub 2022 Jan 19.

Authors

Liuming Xie¹, Zhibing Huang², Hui Meng¹, Xiaoyi Shi¹, Jianhua Xie³

Affiliations

¹ State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China.
² State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China. Electronic address: hzbchem@ncu.edu.cn.
³ State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China.

PMID: 35063487
DOI: 10.1016/j.ijbiomac.2022.01.045

Abstract

A polysaccharide (EMP) was prepared from Monascus purpureus 40,269 through liquid fermentation, and its immunoregulatory effect was investigated to find clues regarding its potential applicability. Structural characterization demonstrated that EMP, with a molecular weight of 83.1 kDa, consists of Xyl, Man, Gal, Ara, Rha, Glc, GalA, and GlcA at a molar ratio of 2.6: 22: 35.1: 7: 1: 29.2: 2.7: 0.6. Immunomodulatory assays involving RAW264.7 cells indicated that EMP exhibits significantly enhanced pinocytic and phagocytic capacities and promotes the secretion of reactive oxygen species, nitric oxide, and cytokines (interleukin-1β, tumor necrosis factor-α, and interleukin-6) by activating RAW264.7 cells. Moreover, EMP is recognized by Toll-like receptor 4 (TLR-4) and has an immunomodulatory effect by activating the mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways in RAW264.7 cells. Collectively, these results demonstrate that EMP could be used as a functional food for immunological reagents.

Keywords: MAPK/NF-κB signaling pathway; Monascus purpureus polysaccharide; Structural characterization.

MeSH terms

Animals
Fermentation
Immunologic Factors / chemistry
Immunologic Factors / pharmacology
MAP Kinase Signaling System* / drug effects
Mice
Mitogen-Activated Protein Kinases / metabolism
Monascus* / chemistry
Monascus* / metabolism
NF-kappa B* / metabolism
Polysaccharides* / chemistry
Polysaccharides* / pharmacology
RAW 264.7 Cells
Receptors, Laminin*
Signal Transduction / drug effects
Toll-Like Receptor 4* / metabolism

Substances

Immunologic Factors
NF-kappa B
Polysaccharides
Receptors, Laminin
Toll-Like Receptor 4
immature laminin receptor protein, mouse
Mitogen-Activated Protein Kinases