Technological advances in super-resolution microscopy to study cellular processes

Charles Bond; Adriana N Santiago-Ruiz; Qing Tang; Melike Lakadamyali

doi:10.1016/j.molcel.2021.12.022

Technological advances in super-resolution microscopy to study cellular processes

Mol Cell. 2022 Jan 20;82(2):315-332. doi: 10.1016/j.molcel.2021.12.022.

Authors

Charles Bond¹, Adriana N Santiago-Ruiz², Qing Tang¹, Melike Lakadamyali³

Affiliations

¹ Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
² Department of Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
³ Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: melikel@pennmedicine.upenn.edu.

Abstract

Since its initial demonstration in 2000, far-field super-resolution light microscopy has undergone tremendous technological developments. In parallel, these developments have opened a new window into visualizing the inner life of cells at unprecedented levels of detail. Here, we review the technical details behind the most common implementations of super-resolution microscopy and highlight some of the recent, promising advances in this field.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Animals
Cell Biology / trends*
Cell Physiological Phenomena*
Diffusion of Innovation
Humans
Image Processing, Computer-Assisted / trends
Microscopy / trends*
Molecular Imaging / trends*
Optical Imaging / trends*
Single Molecule Imaging / trends*

Abstract

Publication types

MeSH terms

Grants and funding