Pedobacter segetis sp. nov., a Novel Bacterium Isolated from Soil

Abstract

A Gram-negative, motile by gliding, rod-shaped, aerobic bacterium, designated SD-b^T, was isolated from a soil sample collected on Dokdo Island, South Korea. A polyphasic approach based on phenotypic, phylogenetic, and genomic analyses was used to characterize the new isolate. Phylogenetic analysis of 16S rRNA gene sequence showed that strain SD-b^T belonged to the family Sphingobacteriaceae and most closely related to Pedobacter psychrophilus P4487A^T (95.9% similarity). The isolate contained MK-7 as the predominant respiratory quinone; its main polar lipid was phosphatidylethanolamine; and the major fatty acids were summed feature 3 (C_16:1 ω7c/C_16:1 ω6c; 32.0%), C_15:0 iso (19.1%), C_17:0 iso 3-OH (8.3%), and C_16:0 (8.2%). The draft genome had a length of 3,842,102 bp with a G+C content of 36.0 mol%, predicting 3282 coding sequences, 3 rRNA genes, 3 ncRNAs, and 36 tRNAs genes. The digital DNA-DNA hybridization and average nucleotide identity values between strain SD-b^T and P. psychrophilus LMG 29436^T were 22.0% and 78.9%, respectively. The results of phenotypic properties, genotypic distinctiveness, and chemotaxonomic features support the discrimination of SD-b^T from its phylogenetic relatives. Pedobacter segetis sp. nov. is therefore proposed with SD-b^T (= KCTC 82351^T = JCM 34283^T) as the type strain.