Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance

Taka-Aki Yano; Taira Kajisa; Masayuki Ono; Yoshiya Miyasaka; Yuichi Hasegawa; Atsushi Saito; Kunihiro Otsuka; Ayuko Sakane; Takuya Sasaki; Koji Yasutomo; Rina Hamajima; Yuta Kanai; Takeshi Kobayashi; Yoshiharu Matsuura; Makoto Itonaga; Takeshi Yasui

doi:10.1038/s41598-022-05036-x

Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance

Sci Rep. 2022 Jan 20;12(1):1060. doi: 10.1038/s41598-022-05036-x.

Authors

Taka-Aki Yano^#¹, Taira Kajisa^#^{2

3}, Masayuki Ono⁴, Yoshiya Miyasaka^#⁴, Yuichi Hasegawa⁴, Atsushi Saito⁴, Kunihiro Otsuka^{2

5}, Ayuko Sakane^{2

6}, Takuya Sasaki⁶, Koji Yasutomo^{2

5}, Rina Hamajima^{7

8}, Yuta Kanai⁷, Takeshi Kobayashi⁷, Yoshiharu Matsuura^{7

9}, Makoto Itonaga⁴, Takeshi Yasui¹⁰

Affiliations

¹ Institute of Post-LED Photonics, Tokushima University, 2-1 Minami-Josanjima, Tokushima, 770-8506, Japan. yano.takaaki@tokushima-u.ac.jp.
² Institute of Post-LED Photonics, Tokushima University, 2-1 Minami-Josanjima, Tokushima, 770-8506, Japan.
³ Graduate School of Interdisciplinary New Science, Toyo University, 2100, Kujirai, Kawagoe, Saitama, 350-8585, Japan.
⁴ JVCKENWOOD Corporation, 58-7, Shinmei-cho, Yokosuka, Kanagawa, 239-8550, Japan.
⁵ Department of Immunology and Parasitology, Graduate School of Medical Sciences, Tokushima University, 3-18-15 Kuramoto, Tokushima, 770-8503, Japan.
⁶ Department of Biochemistry, Graduate School of Medical Sciences, Tokushima University, 3-18-15 Kuramoto, Tokushima, 770-8503, Japan.
⁷ Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
⁸ Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
⁹ Center for Infectious Diseases Education and Research, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
¹⁰ Institute of Post-LED Photonics, Tokushima University, 2-1 Minami-Josanjima, Tokushima, 770-8506, Japan. yasui.takeshi@tokushima-u.ac.jp.

^# Contributed equally.

Abstract

The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing the limit of detection (LOD) is revealed for exceptionally large gold nanoparticles (AuNPs) with diameters of hundreds of nm. SPR enhanced by these large nanoparticles lowered the LOD of SARS-CoV-2 N protein to 85 fM, resulting in the highest SPR detection sensitivity ever obtained for SARS-CoV-2 N protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Coronavirus Nucleocapsid Proteins* / analysis
Coronavirus Nucleocapsid Proteins* / chemistry
Gold / chemistry*
Metal Nanoparticles / chemistry*
Phosphoproteins / analysis
Phosphoproteins / chemistry
SARS-CoV-2 / chemistry*
Surface Plasmon Resonance*

Substances

Coronavirus Nucleocapsid Proteins
Phosphoproteins
nucleocapsid phosphoprotein, SARS-CoV-2
Gold

Abstract

Publication types

MeSH terms

Substances

Grants and funding