κ-Selenocarrageenan, a type of selenized carrageenan polysaccharide, can be degraded by bacteria into oligosaccharides, which has a lower molecular weight and a higher bioavailability. However, research on the microbial degradation of κ-selenocarrageenan is less. In this article, we show that Pseudoalteromonas sp. Xi13, a possibly novel Antarctic bacterium isolated from the floating ice of Southern Ocean, can degrade κ-selenocarrageenan into selenium-oligosaccharides. To gain insights into these biological activities, this bacterium was focused on screening, identification and optimization of submerged fermentation conditions by single-factor experiment. Furthermore, Selenium-oligosaccharides, mainly disaccharides and tetrasaccharides, had a certain inhibitory effect on HeLa cervical cancer cells. Whole genome sequencing and data analysis revealed a plethora of glycoside hydrolase might be involved in κ-selenocarrageenan degradation simultaneously. All told, the recent analysis of above experiment may provide a detailed insight into the characterization, function and catalytic mechanism of Pse sp. Xi13.
Keywords: Antarctica; Complete genome; Degradation; Oligosaccharides; Pseudoalteromonas sp. Xi13; κ-Selenocarrageenan.
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