Endolysosomal Mesoporous Silica Nanoparticle Trafficking along Microtubular Highways

Achraf Noureddine; Michael L Paffett; Stefan Franco; Alfonso E Chan; Sandeep Pallikkuth; Keith Lidke; Rita E Serda

doi:10.3390/pharmaceutics14010056

Endolysosomal Mesoporous Silica Nanoparticle Trafficking along Microtubular Highways

Pharmaceutics. 2021 Dec 27;14(1):56. doi: 10.3390/pharmaceutics14010056.

Authors

Achraf Noureddine¹, Michael L Paffett², Stefan Franco³, Alfonso E Chan³, Sandeep Pallikkuth⁴, Keith Lidke⁴, Rita E Serda^{1

3}

Affiliations

¹ Department of Chemical and Biological Engineering, University of New Mexico, Albuquerque, NM 87131, USA.
² Fluorescence Microscopy Shared Resource, University of New Mexico Comprehensive Cancer Center, Albuquerque, NM 87131, USA.
³ Internal Medicine, University of New Mexico Health Science Center, Albuquerque, NM 87131, USA.
⁴ Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA.

Abstract

This study examines intra- and intercellular trafficking of mesoporous silica nanoparticles along microtubular highways, with an emphasis on intercellular bridges connecting interphase and telophase cells. The study of nanoparticle trafficking within and between cells during all phases of the cell cycle is relevant to payload destination and dilution, and impacts delivery of therapeutic or diagnostic agents. Super-resolution stochastic optical reconstruction and sub-airy unit image acquisition, the latter combined with Huygens deconvolution microscopy, enable single nanoparticle and microtubule resolution. Combined structural and functional data provide enhanced details on biological processes, with an example of mitotic inheritance during cancer cell trivision.

Keywords: Flemming body; cytokinesis; hyperploid cell; mesoporous silica nanoparticle; microtubules; midbody; mitosis; mitotic nanotube; scanning electron microscopy; super-resolution microscopy; tunneling nanotube.