Tolerance of glacial-melt stoneflies (Plecoptera) and morphological responses of chloride cells to stream salinity

Abstract

Aquatic insects within glacial-melt streams are adapted to low dissolved inorganic ion concentrations. Increases in ion concentrations in glacial-melt streams are predicted with increasing air temperatures, which may impact future aquatic insect survival in these streams. We hypothesized that stonefly (Plecoptera) naiads from glacial-melt streams acclimated to different conductivity would differ in survival, median lethal concentrations, and chloride cell responses to elevated conductivity above that expected in our study streams. We conducted field bioassays in remote glacial-melt streams in southwestern China in 2015 and exposed representative stonefly naiads (Chloroperlidae, Nemouridae, Taeniopterygidae) from stream sites differing in conductivity to experimental conductivity ranging from 11 to 20,486 μS/cm for up to 216 h. We examined survivorship, calculated 96-h median lethal concentrations, and measured chloride cell responses with scanning electron microscopy. Chloroperlidae survival after 120 and 216 h did not differ (P > 0.05) among conductivity treatments. The combined Nemouridae/Taeniopterygidae survival after 120 and 216 h was the least (P < 0.05) in conductivity treatments >16,349 μS/cm. Taeniopterygidae survival after 120 h was also the least (P < 0.05) in conductivity treatments >16,349 μS/cm. The 96-h median lethal concentrations did not differ (P > 0.05) between the combined Nemouridae/Taeniopterygidae group (2306 μS/cm) and Taeniopterigydae (2002 μS/cm) and were lower (P < 0.05) than the 96-h median lethal concentration for Chloroperlidae (8167 μS/cm). Chloroperlidae caviform cell number, density, and area decreased (P < 0.05) with increasing conductivity. Taeniopterygidae caviform cell count decreased (P < 0.05) with increasing conductivity, but cell density and area did not. Chloroperlidae and Taeniopterygidae coniform cell characteristics and Nemouridae bulbiform cell characteristics were not affected by conductivity. Our results suggest that Chloroperlidae, Nemouridae, and Taeniopterygidae from glacial-melt streams in China may be able to tolerate moderate increases in conductivity (i.e., 100 to 200 μS/cm).

Keywords: Conductivity; Glacial-melt streams; Osmoregulation; Scanning electron microscopy; Southwest China.