Highly Purified Conjugates of Natural Chlorin with Cobalt Bis(dicarbollide) Nanoclusters for PDT and BNCT Therapy of Cancer

Maria K Fedotova; Maksim N Usachev; Ekaterina V Bogdanova; Ekaterina Diachkova; Yuriy Vasil'ev; Vladimir I Bregadze; Andrey F Mironov; Mikhail A Grin

doi:10.3390/bioengineering9010005

Highly Purified Conjugates of Natural Chlorin with Cobalt Bis(dicarbollide) Nanoclusters for PDT and BNCT Therapy of Cancer

Bioengineering (Basel). 2021 Dec 25;9(1):5. doi: 10.3390/bioengineering9010005.

Authors

Maria K Fedotova¹, Maksim N Usachev¹, Ekaterina V Bogdanova², Ekaterina Diachkova^{3

4}, Yuriy Vasil'ev^{5

6}, Vladimir I Bregadze², Andrey F Mironov¹, Mikhail A Grin¹

Affiliations

¹ Department of Chemistry and Technology of Biologically Active Compounds, Medicinal and Organic Chemistry, Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 86 Vernadsky Avenue, 119571 Moscow, Russia.
² A.N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, 28 Vavilov Str., 119991 Moscow, Russia.
³ Department of Oral Surgery, Borovsky Institute of Dentistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya St. bldg. 8\2, 119435 Moscow, Russia.
⁴ Department of Fundamental Medical Disciplines, Medical Faculty, Moscow Region State University (MRSU), Str. Radio 10 Build 1, 105005 Moscow, Russia.
⁵ Department of Operative Surgery and Topographic Anatomy, I.M. Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya St. bldg. 8\2, 119435 Moscow, Russia.
⁶ Department of Prosthetic Dentistry, Dental Faculty, Kazan State Medical University of the Ministry of Health of Russia, Str. Butlerova 49, 420012 Kazan, Russia.

Abstract

To combine the neutron-capturing and photodynamic properties of boron nanoclusters and derivatives of natural chlorins, respectively, in one molecule, conjugate of chlorin e6 methyl ester with cyclen and dioxane and nitrile derivatives of cobalt bis(dicarbollide) were synthesized. The conditions for the purification of compounds by HPLC were selected since the work with natural compounds is complicated by the production of closely related impurities.

Keywords: UHPLC-HR MS/MS; anticancer; boron neutron capture therapy; nanoconjugate; neutron sensitizer; photodynamic therapy; photosensitizer; preparative chromatography; theranostic.

Grants and funding

19-03-00255/Russian Foundation for Basic Research